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- Elabscience
- 武汉
- E-AB-F1136R1
- 2025年07月21日
- FCM
- Rat
- Mouse
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
该产品可在2-8°C条件下保存34个月。请避免长时间暴露于光线中，并切勿冷冻。
- 克隆性：
Monoclonal
- 标记物：
Elab Bright Violet 510
- 适应物种：
Mouse
- 保质期：
1年
- 级别：
科研
- 库存：
99
- 供应商：
武汉伊莱瑞特生物科技有限公司
- 宿主：
Rat
- 应用范围：
FCM
- 靶点：
CD45
- 抗体英文名：
Elab Bright Violet 510 Anti-Mouse CD45 Antibody[30-F11]
- 抗体名：
Elab Bright Violet 510 Anti-Mouse CD45 Antibody[30-F11]
- 规格：
50 Tests
Elab Bright Violet 510 Anti-Mouse CD45 Antibody[30-F11]
| Alternate Names |
CD45, L-CA, Ly-5, Ptprc, Receptor-type tyrosine-protein phosphatase C, T200
|
| Cellular Localization |
Membrane
|
| Clone No | |
| Isotype |
Rat IgG2b, κ
|
| Leadtime |
咨询
|
| Background |
CD45 is a 180-240 kD glycoprotein also known as the leukocyte common antigen (LCA), T200, or Ly-5. It is a member of the protein tyrosine phosphatase (PTP) family, expressed on all hematopoietic cells except mature erythrocytes and platelets. There are different isoforms of CD45 that arise from variable splicing of exons 4, 5, and 6, which encode A, B, and C determinants, respectively. CD45 plays a key role in TCR and BCR signal transduction. These isoforms are very specific to the activation and maturation state of the cell as well as cell type. The primary ligands for CD45 are galectin-1, CD2, CD3, CD4, TCR, CD22, and Thy-1.
|
| Abbre |
CD45
|
| Swissprot |
P06800
|
| Host |
Rat
|
| Reactivity |
Mouse
|
| Clonality |
Monoclonal
|
| Isotype Control | |
| Applications | |
| Form |
Liquid
|
| Concentration |
5 μL/Test
|
| Conjugation |
Elab Bright Violet 510
|
| Conjugation Information |
Elab Bright Violet 510 is designed to be excited by the violet laser (405 nm) and detected using an optical filter centered near 510 nm (e.g., a 525/50 nm bandpass filter).
|
| Spectrum |
|
| Storage Buffer |
Phosphate buffered solution, pH 7.2, containing 0.09% sodium azide and 1% BSA.
|
| Storage |
This product can be stored at 2-8°C for 12 months. Please protected from prolonged exposure to light and do not freeze.
|
| Expiration Date |
12个月
|
| Shipping |
冰袋
|
| Gene ID |
19264
|
Elab Bright Violet 510 Anti-Mouse CD45 Antibody[30-F11]Elabscience®上线三种新荧光标记（Elab Bright™ Violet 421、Elab Bright™ Violet 510、Elab Bright™ Violet 650）流式抗体53支。目前，Elabscience®已拥有21种标记的流式抗体。
Elab Bright Violet 510 Anti-Mouse CD45 Antibody[30-F11]2-8℃保存12个月
Elab Bright Violet 510 Anti-Mouse CD45 Antibody[30-F11]产品更多产品信息查看欢迎咨询我们和搜索武汉伊莱瑞特Elabscience官网查看。
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文献和实验中。5. 用无菌 PBS 将上述锥形试管填满（至 50 mL)。介绍样品制备。6. 洗涤。在室温、低速制动条件下，以 300 X g 离心分离试管 10 分钟。7. 裂解红细胞（BD 产品目录号：555899，10 x 溶液。在蒸馏水中使用 1 x 溶液。室温孵育 15 分钟。）8. 将 1x106 PBMC 重悬于 20 μL 染色缓冲液中（PBS 含有 5% 正常人血清）。9. 加入抗体：CD117-BV421 (c-kit)、CD7-BV510、CD57- BV605、CD8-BV650
up too rapidly and break. The trick is to gently dry the smear without overheating the slide. 5. Place the slide on a staining rack. Apply the stains on the fixed smear as follows: Flood the slide with crystal violet for 30 sec. Rinse with water
Microscopic Imagery of Mammalian Cells Expressing an Enhanced Green Fluorescent Protein Gone
. Unlike other bioluminescent reporters, the chromophore in GFP is mtrinsic to the primary structure of the protein, and GFP does not require additional factors other than molecular oxygen (see Note 2 ) to fluoresce (6 , 7 ). GFP emits bright green light (λmax = 510 nm
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