4,5-DIFLUORO-2-NITROANILINE

Caspase活性检测系列（分光光度法）检测原理及操作指南

一、检测原理 Caspase (Cysteine-requiring Aspartate Protease)是在细胞凋亡过程中起重要作用的蛋白酶家族。Caspase在正常状态下以酶原的形式存在于胞浆中，没有活性；在细胞发生凋亡阶段，Caspase被激活，活化的Caspase可裂解相应的胞浆胞核底物，最终导致细胞凋亡。 Caspase分光光度法检测试剂盒的原理是将Caspase序列特异性的多肽偶联至黄色发光基团pNA (p-nitroaniline)。当该底物被活化的Caspase剪切后，黄色

Incorporation of Phosphotyrosyl Mimetic 4- (Phosphonodifluoromethyl)phenylalanine (F2Pmp) Into Signal Transduction-Directed Peptides

-stable pTyr mimetics have been developed that retain certain of the chemical and pharmacological properties of pTyr itself. Among these, difluoro-phosphonomethyl phenylalanine (F2 Pmp) has shown widespread utility in a variety of signal transduction

Using Fluorescence-Activated Flow Cytometry to Determine Reactive Oxygen Species Formation and Membrane Lipid Peroxidation in Viable Boar Spermatozoa

probe 4,4-difluoro-5-(4-phenyl-1,3-butadienyl)-4-bora-3a,4a-diaza-s-indacene-3-undecanoic acid, C11 BODIPY581/591 (BODIPY). ROS was detected by red fluorescence emission from oxidization of HE and membrane lipid peroxidation was detected by green