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文献和实验different-length vectors. Don't use less than 8 ng or so for fragments smaller than 0.5 kb. In the case of small fragments circularization occurs and removes the F from the rxn. F Used in Standard Ligation
Standard hybridization technique
The standard solution typically used for both pre-hybridisation and hybridisation is based
of small fragments circularization occurs and removes the F from the rxn. F Used in Standard Ligation vector (50 ng, 3kb
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