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- 详细信息
- 文献和实验
- 技术资料
- 库存:
100
- 供应商:
LSM BIO
- 检测范围:
0.78-50ng/ml
- 检测方法:
夹心法
- 应用:
利用ELISA方法体外定量检测鸡血清,血浆等样本中目标蛋白的浓度
- 适应物种:
鸡
- 标记物:
Chicken Importin subunit alpha-1,KPNA1
- 样本:
鸡的血浆,血清,细胞上清等
- 灵敏度:
0.469ng/ml
- 规格:
96tests
Chicken Importin subunit alpha- 1, KPNA1 ELISA KIT
ELI-38453c
FOR LABORATORY RESEARCH USE ONLY. NOT FOR
USE IN DIAGNOSTIC PROCEDURES.
Chicken Importin subunit alpha- 1, KPNA1 ELISA KIT is for the quantitative in vitro determination of Chicken KPNA1 concentrations in serum - plasma - tissue homogenates - other biological fluids.
INTENDED USE AND TEST PRINCIPLE
This Chicken Importin subunit alpha- 1, KPNA1 ELISA KIT is intended Laboratory for Research use only and it is not for use in diagnostic or therapeutic procedures. The Stop Solution changes the color from blue to yellow and the intensity of the color is measured at 450 nm using a spectrophotometer. In order to measure the concentration of Chicken KPNA1 in the sample, this Chicken Importin subunit alpha- 1, KPNA1 ELISA KIT includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of Optical Density versus Chicken KPNA1 concentration. The concentration of Chicken KPNA1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
REAGENTS PROVIDED
All reagents provided are stored at 2-8 degree. Refer to the expiration date on the label.
MICROTITER PLATE 12x8strips
STANDARD 6x0.3ml vial
SAMPLE DILUENT 6.0ml
ENZYME CONJUGATE 10.0ml
WASH SOLUTION 25ml
SUBSTRATE A 6.0ml
SUBSTRATE B 6.0ml
STOP SOLUTION 6.0ml
Closure plate membrane 2
User manual 1
Sealed bags 1
Note:
1.This package insert must be read in its entirety before using this product.
2.This ELISA Kit is used for lab research only!
"(本产品仅供体外科研用途,不可用于临床诊断!)
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文献和实验。当我们将已知基因作为诱饵,在选定的cDNA文库中筛选与诱饵蛋白相互作用的蛋白,从筛选到的阳性酵母菌株中可以分离得到AD-LIBRARY载体,并从载体中进一步克隆得到随机插入的cDNA片段,并对该片段的编码序列在GENEBANK中进行比较,研究与已知基因在生物学功能上的联系。 另外,也可作为研究已知基因的新功能或多个筛选到的已知基因之间功能相关的主要方法。例如:Engelender等人以神经末端蛋白alpha-synuclein蛋白为诱饵蛋白,利用酵母
。当我们将已知基因作为诱饵,在选定的cDNA文库中筛选与诱饵蛋白相互作用的蛋白�由秆〉降难粜越湍妇�曛锌梢苑掷氲玫�D-LIBRARY载体,并从载体中进一步克隆得到随机插入的cDNA片段,并对该片段的编码序列在GENEBANK中进行比较,研究与已知基因在生物学功能上的联系。另外,也可作为研究已知基因的新功能或多个筛选到的已知基因之间功能相关的主要方法。例如:Engelender等人以神经末端蛋白alpha-synuclein 蛋白为诱饵蛋白,利用酵母双杂交CLONTECH MATCHMARKER
Antigen Design Sera Purification Tech Sheet
for antibody generation. Algorithms for predicting protein characteristics such as hydrophilicity/hydrophobicity and secondary structure regions such as alpha-helix, beta-sheet and beta-turn aid selection of a potentially exposed, immunogenic internal
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