- ￥3480
- LSM Bio
- 0.78-50ng/ml
- 0.469ng/ml
- 进口
- ELI-29776c
- 2025年07月16日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
100
- 供应商：
LSM BIO
- 检测范围：
0.78-50ng/ml
- 检测方法：
夹心法
- 应用：
利用ELISA方法体外定量检测鸡血清，血浆等样本中目标蛋白的浓度
- 适应物种：
鸡
- 标记物：
Chicken Septin-2,SEPT2
- 样本：
鸡的血浆，血清，细胞上清等
- 灵敏度：
0.469ng/ml
- 规格：
96tests
Chicken Septin- 2, SEPT2 ELISA KIT
ELI-29776c
FOR LABORATORY RESEARCH USE ONLY. NOT FOR
USE IN DIAGNOSTIC PROCEDURES.
Chicken Septin- 2, SEPT2 ELISA KIT is for the quantitative in vitro determination of Chicken SEPT2 concentrations in serum - plasma - tissue homogenates - other biological fluids.
INTENDED USE AND TEST PRINCIPLE
This Chicken Septin- 2, SEPT2 ELISA KIT is intended Laboratory for Research use only and it is not for use in diagnostic or therapeutic procedures. The Stop Solution changes the color from blue to yellow and the intensity of the color is measured at 450 nm using a spectrophotometer. In order to measure the concentration of Chicken SEPT2 in the sample, this Chicken Septin- 2, SEPT2 ELISA KIT includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of Optical Density versus Chicken SEPT2 concentration. The concentration of Chicken SEPT2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
REAGENTS PROVIDED
All reagents provided are stored at 2-8 degree. Refer to the expiration date on the label.
MICROTITER PLATE 12x8strips
STANDARD 6x0.3ml vial
SAMPLE DILUENT 6.0ml
ENZYME CONJUGATE 10.0ml
WASH SOLUTION 25ml
SUBSTRATE A 6.0ml
SUBSTRATE B 6.0ml
STOP SOLUTION 6.0ml
Closure plate membrane 2
User manual 1
Sealed bags 1
Note:
1.This package insert must be read in its entirety before using this product.
2.This ELISA Kit is used for lab research only!
"(本产品仅供体外科研用途，不可用于临床诊断!)
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文献和实验说明书为准。 保存条件及有效期 纤维素 fitc流式ZNF286IHC、FACS、WB、E 1．试剂盒保存：；2-8℃。 2．有效期：6 个月 人一氧化氮(NO)酶联试剂盒说明书 2-甲氧乙基酯 fitc流式ZNF537IHC、FACS、WB、ELISA Rabbit polyclonal to ZNF537 antibody 乙二胺 fitc流式ZNF5IHC、FACS、WB、ELISA Rabbit
多篇 SCI 发现用 RIPA 提蛋白存在问题，我们该如何应对？
相互作用）及其他成分。虽然 RIPA 裂解液经过不断改良使用，但是 RIPA 提取蛋白通常会产生两个不同的部分：即 RIPA 可溶组分和 RIPA 不可溶组分。 一般来说，只有 RIPA 可溶组分被用于下游实验，而 RIPA 不可溶组分被丢弃。这样提取的蛋白在使用中会存在哪些问题呢？先来看些文献数据：研究发现，不同的蛋白在 RIPA 裂解液中溶解性不同，例如 F9 中的纤维连接蛋白不溶于 RIPA 裂解液 [2] 。而小鼠额叶样品中在 RIPA 可溶组分和非可溶性组分中含有相同量的 Septin
,and the cell pellets were frozen and stored at –80ºC. Lysis Lysis was accomplished with B-PER reagent supplemented with 1/50 vol 10-mg/ml chicken egg-white lysozyme (stored at –80ºC in aliquots),1/125 vol 5-mg/ml DNaseI (in 20 mM sodium acetate,pH adjusted
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