- ￥3480
- LSM Bio
- 0.78-50ng/ml
- 0.469ng/ml
- 进口
- ELI-26670c
- 2025年07月14日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
100
- 供应商：
LSM BIO
- 检测范围：
0.78-50ng/ml
- 检测方法：
夹心法
- 应用：
利用ELISA方法体外定量检测鸡血清，血浆等样本中目标蛋白的浓度
- 适应物种：
鸡
- 标记物：
Chicken Spliceosome RNA helicase DDX39B,DDX39B
- 样本：
鸡的血浆，血清，细胞上清等
- 灵敏度：
0.469ng/ml
- 规格：
96tests
Chicken Spliceosome RNA helicase DDX39B, DDX39B ELISA KIT
ELI-26670c
FOR LABORATORY RESEARCH USE ONLY. NOT FOR
USE IN DIAGNOSTIC PROCEDURES.
Chicken Spliceosome RNA helicase DDX39B, DDX39B ELISA KIT is for the quantitative in vitro determination of Chicken DDX39B concentrations in serum - plasma - tissue homogenates - other biological fluids.
INTENDED USE AND TEST PRINCIPLE
This Chicken Spliceosome RNA helicase DDX39B, DDX39B ELISA KIT is intended Laboratory for Research use only and it is not for use in diagnostic or therapeutic procedures. The Stop Solution changes the color from blue to yellow and the intensity of the color is measured at 450 nm using a spectrophotometer. In order to measure the concentration of Chicken DDX39B in the sample, this Chicken Spliceosome RNA helicase DDX39B, DDX39B ELISA KIT includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of Optical Density versus Chicken DDX39B concentration. The concentration of Chicken DDX39B in the samples is then determined by comparing the O.D. of the samples to the standard curve.
REAGENTS PROVIDED
All reagents provided are stored at 2-8 degree. Refer to the expiration date on the label.
MICROTITER PLATE 12x8strips
STANDARD 6x0.3ml vial
SAMPLE DILUENT 6.0ml
ENZYME CONJUGATE 10.0ml
WASH SOLUTION 25ml
SUBSTRATE A 6.0ml
SUBSTRATE B 6.0ml
STOP SOLUTION 6.0ml
Closure plate membrane 2
User manual 1
Sealed bags 1
Note:
1.This package insert must be read in its entirety before using this product.
2.This ELISA Kit is used for lab research only!
"(本产品仅供体外科研用途，不可用于临床诊断!)
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文献和实验♦ 适用范围：适用于快速提取植物组织细胞总RNA本试剂盒在室温储存12个月不影响使用效果。♦ 储存事项：1. 所有的溶液应该是澄清的，如果环境温度低时溶液可能形成沉淀，此时不应该直接使用，可在37℃水浴加热几分钟，即可恢复澄清。2. 不合适的储存于低温（4℃或者－20℃）会造成溶液沉淀，影响使用效果，因此运输和储存均在室温下（15℃－25℃）进行。3. 避免试剂长时间暴露于空气中产生挥发、氧化、PH值变化，各溶液使用后应及时盖紧盖子。♦ 产品介绍：独特的裂解液/β-巯基乙醇迅速裂解细胞和灭
提取总RNA 和基因组DNA 为一次制备。本试剂盒所有试剂在室温贮存2 年不影响使用效果。Buffer R-A：细胞裂解液，室温密闭贮存。Buffer R-B：蛋白质变性剂，室温密闭贮。Buffer R-C：分相溶液，室温密闭贮存。Buffer R-D：分相溶液，室温密闭贮存。Buffer R-E：RNA 释放溶液，使用前检查溶液是否有沉淀析出，如有沉淀请于37℃温育溶解后再使用。室温密闭贮存。RNase-free Water：RNA 溶解溶液，室温密闭贮存。Buffer TE：10mM Tris
,and the cell pellets were frozen and stored at –80ºC. Lysis Lysis was accomplished with B-PER reagent supplemented with 1/50 vol 10-mg/ml chicken egg-white lysozyme (stored at –80ºC in aliquots),1/125 vol 5-mg/ml DNaseI (in 20 mM sodium acetate,pH adjusted
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