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- 详细信息
- 文献和实验
- 技术资料
- 库存:
100
- 供应商:
LSM BIO
- 检测范围:
0.78-50ng/ml
- 检测方法:
夹心法
- 应用:
利用ELISA方法体外定量检测鸡血清,血浆等样本中目标蛋白的浓度
- 适应物种:
鸡
- 标记物:
Chicken Nuclear factor 1 X-type,NFIX
- 样本:
鸡的血浆,血清,细胞上清等
- 灵敏度:
0.469ng/ml
- 规格:
96tests
Chicken Nuclear factor 1 X- type, NFIX ELISA KIT
ELI-22187c
FOR LABORATORY RESEARCH USE ONLY. NOT FOR
USE IN DIAGNOSTIC PROCEDURES.
Chicken Nuclear factor 1 X- type, NFIX ELISA KIT is for the quantitative in vitro determination of Chicken NFIX concentrations in serum - plasma - tissue homogenates - other biological fluids.
INTENDED USE AND TEST PRINCIPLE
This Chicken Nuclear factor 1 X- type, NFIX ELISA KIT is intended Laboratory for Research use only and it is not for use in diagnostic or therapeutic procedures. The Stop Solution changes the color from blue to yellow and the intensity of the color is measured at 450 nm using a spectrophotometer. In order to measure the concentration of Chicken NFIX in the sample, this Chicken Nuclear factor 1 X- type, NFIX ELISA KIT includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of Optical Density versus Chicken NFIX concentration. The concentration of Chicken NFIX in the samples is then determined by comparing the O.D. of the samples to the standard curve.
REAGENTS PROVIDED
All reagents provided are stored at 2-8 degree. Refer to the expiration date on the label.
MICROTITER PLATE 12x8strips
STANDARD 6x0.3ml vial
SAMPLE DILUENT 6.0ml
ENZYME CONJUGATE 10.0ml
WASH SOLUTION 25ml
SUBSTRATE A 6.0ml
SUBSTRATE B 6.0ml
STOP SOLUTION 6.0ml
Closure plate membrane 2
User manual 1
Sealed bags 1
Note:
1.This package insert must be read in its entirety before using this product.
2.This ELISA Kit is used for lab research only!
"(本产品仅供体外科研用途,不可用于临床诊断!)
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文献和实验为了获得全长的cDNA 5’及3’末端序列,许多研究人员使用了称之为cDNA末端快速扩增,或RACE的方法。传统的RACE方法得到的PCR产物含有全长及断裂的cDNA产物。GeneRacer™试剂盒确保您只得到含有全长cDNA末端的完整序列,是您得到更高效的结果并节省您的时间GeneRacer™的优点:确定一个基因的全长序列对于研究异源转录起始和得到用于蛋白表达的开放阅读框至关重要。GeneRacer™是一种高级RACE技术,提高了扩增全长cDNA末端序列的效率。使用GeneRacer™试剂盒
and 1-3 dmin present in most cells.Viruses: ELISA: reverse transcriptase positive.Cell line:BA/F3.Cell type:mouse pro B cells.Origin:IL-3 dependent murine pro B cell line established from peripheralblood; apparently derived from BALB/c mouse.Morphology
DETECTION OF ß-GALACTOSIDASE AND ALKALINE PHOSPHATASE ACTIVITIES IN TISSUE
reaction buffer can be stored for at least one year at room temperature in foil-covered container. X-gal stock(40X) 40 mg/ml X-gal in dimethylformamide Store at -200 C in foil covered glass container. Rinse many times in PBS
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