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- 详细信息
- 文献和实验
- 技术资料
- 库存:
100
- 供应商:
LSM BIO
- 检测范围:
0.78-50ng/ml
- 检测方法:
夹心法
- 应用:
利用ELISA方法体外定量检测牛血清,血浆等样本中目标蛋白的浓度
- 适应物种:
牛
- 标记物:
Bovine CCAAT/enhancer-binding protein delta,CEBPD
- 样本:
牛的血浆,血清,细胞上清等
- 灵敏度:
0.469ng/ml
- 规格:
96tests
Bovine CCAAT/enhancer- binding protein delta, CEBPD ELISA KIT
Product Name:Bovine CCAAT/enhancer- binding protein delta, CEBPD ELISA KIT
Packing:96T
Catalog No.:ELI-50846b
Gene Name:Bovine CCAAT(enhancer-binding protein delta, CEBPD
Detect Range:0.156-10ng/ml
Sensitivity:0.094ng/ml
Target Protein Name:Bovine CCAAT(enhancer-binding protein delta, CEBPD
Alternative Name:CEBPD,Bovine CCAAT(enhancer-binding protein delta, CEBPD
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Bovine CCAAT/enhancer- binding protein delta, CEBPD ELISA KIT allows for the in vitro quantitative determination of Bovine CCAAT(enhancer-binding protein delta, CEBPD concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Bovine CCAAT/enhancer- binding protein delta, CEBPD ELISA KIT has been pre-coated with an Bovine CCAAT(enhancer-binding protein delta, CEBPD antibody specific to Bovine CCAAT(enhancer-binding protein delta, CEBPD .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Bovine CCAAT(enhancer-binding protein delta, CEBPD and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Bovine CCAAT(enhancer-binding protein delta, CEBPD, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Bovine CCAAT(enhancer-binding protein delta, CEBPD in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验Mapping Protein Distributions on Polytene Chromosomes by Immunostaining
stock solution Nutrient-rich fly medium Phosphate-buffered saline (PBS; pH 7.5) Poly-L-lysine solution (0.1% [w/v] in H2 O; Sigma P8920) Sodium phosphate buffer (10 mM, pH 6.8) Triton X-100 VECTASTAIN Elite ABC Kit
Solid Phase Assays for Studying ECM Protein-Protein Interactions
of an enzyme-linked immunosorbent assay (ELISA) plate (solid phase). The plate is then blocked using bovine serum albumin (BSA), and biotin-labeled protein B (here termed ’ligand’) is added. After washing the wells to remove unbound ligand, bound ligand
Substrate Tablete(antibody developed in goat) St. Louis,Mo 63178, USAAffinity isolated antibody adsorbed Lot # 043H6178With bovine horse and human serum protein Cat # 0h176Lot # 055H4835Lot # A-2179Tween 80Laboratories ,Detroit,Michigan ,USA1 drop of Tween
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