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- 详细信息
- 文献和实验
- 技术资料
- 库存:
100
- 供应商:
LSM BIO
- 检测范围:
0.78-50ng/ml
- 检测方法:
夹心法
- 应用:
利用ELISA方法体外定量检测鸡血清,血浆等样本中目标蛋白的浓度
- 适应物种:
鸡
- 标记物:
Chicken 26S protease regulatory subunit 4,PSMC1
- 样本:
鸡的血浆,血清,细胞上清等
- 灵敏度:
0.469ng/ml
- 规格:
96tests
Chicken 26S protease regulatory subunit 4, PSMC1 ELISA KIT
ELI-15680c
FOR LABORATORY RESEARCH USE ONLY. NOT FOR
USE IN DIAGNOSTIC PROCEDURES.
Chicken 26S protease regulatory subunit 4, PSMC1 ELISA KIT is for the quantitative in vitro determination of Chicken PSMC1 concentrations in serum - plasma - tissue homogenates - other biological fluids.
INTENDED USE AND TEST PRINCIPLE
This Chicken 26S protease regulatory subunit 4, PSMC1 ELISA KIT is intended Laboratory for Research use only and it is not for use in diagnostic or therapeutic procedures. The Stop Solution changes the color from blue to yellow and the intensity of the color is measured at 450 nm using a spectrophotometer. In order to measure the concentration of Chicken PSMC1 in the sample, this Chicken 26S protease regulatory subunit 4, PSMC1 ELISA KIT includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of Optical Density versus Chicken PSMC1 concentration. The concentration of Chicken PSMC1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
REAGENTS PROVIDED
All reagents provided are stored at 2-8 degree. Refer to the expiration date on the label.
MICROTITER PLATE 12x8strips
STANDARD 6x0.3ml vial
SAMPLE DILUENT 6.0ml
ENZYME CONJUGATE 10.0ml
WASH SOLUTION 25ml
SUBSTRATE A 6.0ml
SUBSTRATE B 6.0ml
STOP SOLUTION 6.0ml
Closure plate membrane 2
User manual 1
Sealed bags 1
Note:
1.This package insert must be read in its entirety before using this product.
2.This ELISA Kit is used for lab research only!
"(本产品仅供体外科研用途,不可用于临床诊断!)
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文献和实验In Vitro Reconstitution of CFTR Biogenesis and Degradation
is recognized by a large multicatalytic protease, the 26S proteasome. This proteolytic machine is formed through the ATP-dependent association of two multimeric components, the 20S proteasome and the 19S regulatory subunit. The 19S subunit recognizes
-femtomolar level if enough radioactivity can be incorporated into the phosphoprotein of interest. In our hands, this can be as little as 1,000 cpm (not ideal). Briefly, a 32 P-labeled protein is digested with a protease and the resulting phosphopeptides
[资源] 所有的看家基因(housekeeping genes)列表+引物设计服务
IV isoform 1 (COX4I1), nuclear gene encoding mitochondrial protein, mRNA 2738 NM_004890 Homo sapiens sperm associated antigen 7 (SPAG7), mRNA 618 NM_002812 Homo sapiens proteasome (prosome, macropain) 26S subunit, non-ATPase, 8 (PSMD8), mRNA 942
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