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- 详细信息
- 文献和实验
- 技术资料
- 库存:
100
- 供应商:
LSM BIO
- 检测范围:
0.78-50ng/ml
- 检测方法:
夹心法
- 应用:
利用ELISA方法体外定量检测鸡血清,血浆等样本中目标蛋白的浓度
- 适应物种:
鸡
- 标记物:
Chicken G1/S-specific cyclin-E1,CCNE1
- 样本:
鸡的血浆,血清,细胞上清等
- 灵敏度:
0.469ng/ml
- 规格:
96tests
Chicken G1/S- specific cyclin- E1, CCNE1 ELISA KIT
ELI-11399c
FOR LABORATORY RESEARCH USE ONLY. NOT FOR
USE IN DIAGNOSTIC PROCEDURES.
Chicken G1/S- specific cyclin- E1, CCNE1 ELISA KIT is for the quantitative in vitro determination of Chicken CCNE1 concentrations in serum - plasma - tissue homogenates - other biological fluids.
INTENDED USE AND TEST PRINCIPLE
This Chicken G1/S- specific cyclin- E1, CCNE1 ELISA KIT is intended Laboratory for Research use only and it is not for use in diagnostic or therapeutic procedures. The Stop Solution changes the color from blue to yellow and the intensity of the color is measured at 450 nm using a spectrophotometer. In order to measure the concentration of Chicken CCNE1 in the sample, this Chicken G1/S- specific cyclin- E1, CCNE1 ELISA KIT includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of Optical Density versus Chicken CCNE1 concentration. The concentration of Chicken CCNE1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
REAGENTS PROVIDED
All reagents provided are stored at 2-8 degree. Refer to the expiration date on the label.
MICROTITER PLATE 12x8strips
STANDARD 6x0.3ml vial
SAMPLE DILUENT 6.0ml
ENZYME CONJUGATE 10.0ml
WASH SOLUTION 25ml
SUBSTRATE A 6.0ml
SUBSTRATE B 6.0ml
STOP SOLUTION 6.0ml
Closure plate membrane 2
User manual 1
Sealed bags 1
Note:
1.This package insert must be read in its entirety before using this product.
2.This ELISA Kit is used for lab research only!
"(本产品仅供体外科研用途,不可用于临床诊断!)
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文献和实验Maintenance of Chicken Embryonic Stem Cells In Vitro
as a fusion protein linked to glutathione S-transferase (GST) and purified to greater than 90% purity in two chro-matography stages, the first an affinity step using the GST tail, which was cleaved before further purification by gel chromatography. Chicken ES
RB 是一种 105Kb 的核内磷蛋白,它对细胞周期也有一定的影响。保持在 G0/G1 期的静止期细胞中 RB 是未磷酸化的,在细胞周期中它被周期蛋白 CDK ( cyclin-CDK )复合物磷酸化。大部分情况是发生在 G1 期末,一直延伸到 G2 期末,在有些分裂中是去磷酸化。 RB 在非磷酸化状态才能和几种蛋白如 SV40 T 抗原等特异结合。这些相互作用只发生在细胞同期的特定阶段(在 S 期前) RB- 经磷酸化就会释放这些蛋白。 靶蛋白中包括一组
Enzyme-Linked ImmunoSorbent Assay (ELISA) for NGF
apparatus. Aspirate with pasteur pipets as before. A triple wash is required, this time using the Nunc washer to fill and aspirate each row 3x simultaneously before moving on to the next row. Dry the wells by removing the remaining liquid with pasteur pipets
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