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- 详细信息
- 文献和实验
- 技术资料
- 库存:
100
- 供应商:
LSM BIO
- 检测范围:
0.78-50ng/ml
- 检测方法:
夹心法
- 应用:
利用ELISA方法体外定量检测鸡血清,血浆等样本中目标蛋白的浓度
- 适应物种:
鸡
- 标记物:
Chicken G1/S-specific cyclin-D2,CCND2
- 样本:
鸡的血浆,血清,细胞上清等
- 灵敏度:
0.469ng/ml
- 规格:
96tests
Chicken G1/S- specific cyclin- D2, CCND2 ELISA KIT
ELI-02109c
FOR LABORATORY RESEARCH USE ONLY. NOT FOR
USE IN DIAGNOSTIC PROCEDURES.
Chicken G1/S- specific cyclin- D2, CCND2 ELISA KIT is for the quantitative in vitro determination of Chicken CCND2 concentrations in serum - plasma - tissue homogenates - other biological fluids.
INTENDED USE AND TEST PRINCIPLE
This Chicken G1/S- specific cyclin- D2, CCND2 ELISA KIT is intended Laboratory for Research use only and it is not for use in diagnostic or therapeutic procedures. The Stop Solution changes the color from blue to yellow and the intensity of the color is measured at 450 nm using a spectrophotometer. In order to measure the concentration of Chicken CCND2 in the sample, this Chicken G1/S- specific cyclin- D2, CCND2 ELISA KIT includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of Optical Density versus Chicken CCND2 concentration. The concentration of Chicken CCND2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
REAGENTS PROVIDED
All reagents provided are stored at 2-8 degree. Refer to the expiration date on the label.
MICROTITER PLATE 12x8strips
STANDARD 6x0.3ml vial
SAMPLE DILUENT 6.0ml
ENZYME CONJUGATE 10.0ml
WASH SOLUTION 25ml
SUBSTRATE A 6.0ml
SUBSTRATE B 6.0ml
STOP SOLUTION 6.0ml
Closure plate membrane 2
User manual 1
Sealed bags 1
Note:
1.This package insert must be read in its entirety before using this product.
2.This ELISA Kit is used for lab research only!
"(本产品仅供体外科研用途,不可用于临床诊断!)
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文献和实验3 篇 Nature 连发,解决世纪难题,周期蛋白的毁灭调控蕴含癌症患者的新生
的是两条平行的信号通路,MYC 通路和 cyclin D-CDK-RB 通路。在癌症中 MYC 和 cyclin D-CDK-RB 都是失调的,这与增加的基因组不稳定性相关。虽然已知自噬肿瘤抑制蛋白 AMBRA1 与细胞增殖的控制有关,但其潜在的分子机制尚不清楚。图片来源:Nature Maiani 及其同事通过细胞和分子方法的结合以及体内模型,发现 AMBRA1 通过介导 cyclin D 降解,从而调节其丰度。此外,AMBRA1 是 G1 期到 S 期过度的上游主要调节因子,通过控制从 G1 期
Maintenance of Chicken Embryonic Stem Cells In Vitro
as a fusion protein linked to glutathione S-transferase (GST) and purified to greater than 90% purity in two chro-matography stages, the first an affinity step using the GST tail, which was cleaved before further purification by gel chromatography. Chicken ES
Enzyme-Linked ImmunoSorbent Assay (ELISA) for NGF
apparatus. Aspirate with pasteur pipets as before. A triple wash is required, this time using the Nunc washer to fill and aspirate each row 3x simultaneously before moving on to the next row. Dry the wells by removing the remaining liquid with pasteur pipets
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