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- 详细信息
- 文献和实验
- 技术资料
- 库存:
100
- 供应商:
LSM BIO
- 检测范围:
0.78-50ng/ml
- 检测方法:
夹心法
- 应用:
利用ELISA方法体外定量检测鸡血清,血浆等样本中目标蛋白的浓度
- 适应物种:
鸡
- 标记物:
Chicken Thymidine kinase,cytosolic,TK1
- 样本:
鸡的血浆,血清,细胞上清等
- 灵敏度:
0.469ng/ml
- 规格:
96tests
Chicken Thymidine kinase, cytosolic, TK1 ELISA KIT
ELI-06769c
FOR LABORATORY RESEARCH USE ONLY. NOT FOR
USE IN DIAGNOSTIC PROCEDURES.
Chicken Thymidine kinase, cytosolic, TK1 ELISA KIT is for the quantitative in vitro determination of Chicken TK1 concentrations in serum - plasma - tissue homogenates - other biological fluids.
INTENDED USE AND TEST PRINCIPLE
This Chicken Thymidine kinase, cytosolic, TK1 ELISA KIT is intended Laboratory for Research use only and it is not for use in diagnostic or therapeutic procedures. The Stop Solution changes the color from blue to yellow and the intensity of the color is measured at 450 nm using a spectrophotometer. In order to measure the concentration of Chicken TK1 in the sample, this Chicken Thymidine kinase, cytosolic, TK1 ELISA KIT includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of Optical Density versus Chicken TK1 concentration. The concentration of Chicken TK1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
REAGENTS PROVIDED
All reagents provided are stored at 2-8 degree. Refer to the expiration date on the label.
MICROTITER PLATE 12x8strips
STANDARD 6x0.3ml vial
SAMPLE DILUENT 6.0ml
ENZYME CONJUGATE 10.0ml
WASH SOLUTION 25ml
SUBSTRATE A 6.0ml
SUBSTRATE B 6.0ml
STOP SOLUTION 6.0ml
Closure plate membrane 2
User manual 1
Sealed bags 1
Note:
1.This package insert must be read in its entirety before using this product.
2.This ELISA Kit is used for lab research only!
"(本产品仅供体外科研用途,不可用于临床诊断!)
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文献和实验或G/C高含量的transcripts也适用 • 由于其天然结构和真核生物来源,蛋白溶解度高 • 无动物成分,无血清 • 可线性扩大产量 • 对于膜蛋白不需要添加人工脂质体 • 可诱导形成二硫键 • 低成本,平均每个反应产出10μg蛋白,每个试剂盒提供50个反应 已验证可成功表达:Kinase, Enzyme, Transporter, Receptor and GPCR proteins, from 10kDa to over 220kDa CFPE700-新一代无细胞蛋白表达试剂盒
Screening for Chemical Mutagens Using the Mouse Lymphoma Assay
The mouse lymphoma assay (MLA) using the thymidine kinase (Tk1 ) gene is widely used to detect the potential genotoxicity of a wide variety of chemical agents. The assay is recommended as a part of the core battery of genetic toxicology tests
tongenjuan 我想要研究肿瘤细胞在药物及照射的情况下,其细胞的生长情况。考虑到一块板上不能同时做药物及照射,因为照射一部分孔时不能保证药物组不受射线影响;而且如果分为2块版的话,只能分别检测,结果比较就没有意义了。因此想询问一下除了MTT法,还有没有其他的方法可以检测到细胞生长抑制情况 湘洋剑客 貌似必须两块板 不用MTT,CCK8也行 流式ELISA蛋白
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