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- 详细信息
- 文献和实验
- 技术资料
- 库存:
100
- 供应商:
LSM BIO
- 检测范围:
0.78-50ng/ml
- 检测方法:
夹心法
- 应用:
利用ELISA方法体外定量检测牛血清,血浆等样本中目标蛋白的浓度
- 适应物种:
牛
- 标记物:
Bovine Receptor expression-enhancing protein 2,REEP2
- 样本:
牛的血浆,血清,细胞上清等
- 灵敏度:
0.469ng/ml
- 规格:
96tests
Bovine Receptor expression- enhancing protein 2, REEP2 ELISA KIT
Product Name:Bovine Receptor expression- enhancing protein 2, REEP2 ELISA KIT
Packing:96T
Catalog No.:ELI-22529b
Gene Name:Bovine Receptor expression-enhancing protein 2, REEP2
Detect Range:0.781-50ng/ml
Sensitivity:0.469ng/ml
Target Protein Name:Bovine Receptor expression-enhancing protein 2, REEP2
Alternative Name:REEP2,Bovine Receptor expression-enhancing protein 2, REEP2
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Bovine Receptor expression- enhancing protein 2, REEP2 ELISA KIT allows for the in vitro quantitative determination of Bovine Receptor expression-enhancing protein 2, REEP2 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Bovine Receptor expression- enhancing protein 2, REEP2 ELISA KIT has been pre-coated with an Bovine Receptor expression-enhancing protein 2, REEP2 antibody specific to Bovine Receptor expression-enhancing protein 2, REEP2 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Bovine Receptor expression-enhancing protein 2, REEP2 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Bovine Receptor expression-enhancing protein 2, REEP2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Bovine Receptor expression-enhancing protein 2, REEP2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验Strategies to Optimize Protein Expression in E. coli
Peng, L., Xu, Z.N., Fang, X.M., Wang, F., Yang, S., and Cen, P.L. 2004. Preferential codons enhancing the expression level of human beta‐defensin‐2 in recombinant Escherichia coli. Protein Pept. Lett. 11:339
Detecting Low‐Affinity Extracellular Protein Interactions Using Protein Microarrays
the throughput of assays capable of detecting transient extracellular protein interactions. These methodological improvements now permit screening for novel extracellular receptor?ligand interactions on a genome?wide scale. Curr. Protoc. Protein Sci. 72:27.5.1?27
Identification of Protein Interactions by Far Western Analysis
protein of interest cloned into an in vitro expression vector In vitro transcription/translation kit (Promega) 10 mCi/ml 35
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