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- 详细信息
- 文献和实验
- 技术资料
- 库存:
100
- 供应商:
LSM BIO
- 检测范围:
0.78-50ng/ml
- 检测方法:
夹心法
- 应用:
利用ELISA方法体外定量检测鸡血清,血浆等样本中目标蛋白的浓度
- 适应物种:
鸡
- 标记物:
Chicken DNA nucleotidylexotransferase,DNTT
- 样本:
鸡的血浆,血清,细胞上清等
- 灵敏度:
0.469ng/ml
- 规格:
96tests
Chicken DNA nucleotidylexotransferase, DNTT ELISA KIT
ELI-05095c
FOR LABORATORY RESEARCH USE ONLY. NOT FOR
USE IN DIAGNOSTIC PROCEDURES.
Chicken DNA nucleotidylexotransferase, DNTT ELISA KIT is for the quantitative in vitro determination of Chicken DNTT concentrations in serum - plasma - tissue homogenates - other biological fluids.
INTENDED USE AND TEST PRINCIPLE
This Chicken DNA nucleotidylexotransferase, DNTT ELISA KIT is intended Laboratory for Research use only and it is not for use in diagnostic or therapeutic procedures. The Stop Solution changes the color from blue to yellow and the intensity of the color is measured at 450 nm using a spectrophotometer. In order to measure the concentration of Chicken DNTT in the sample, this Chicken DNA nucleotidylexotransferase, DNTT ELISA KIT includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of Optical Density versus Chicken DNTT concentration. The concentration of Chicken DNTT in the samples is then determined by comparing the O.D. of the samples to the standard curve.
REAGENTS PROVIDED
All reagents provided are stored at 2-8 degree. Refer to the expiration date on the label.
MICROTITER PLATE 12x8strips
STANDARD 6x0.3ml vial
SAMPLE DILUENT 6.0ml
ENZYME CONJUGATE 10.0ml
WASH SOLUTION 25ml
SUBSTRATE A 6.0ml
SUBSTRATE B 6.0ml
STOP SOLUTION 6.0ml
Closure plate membrane 2
User manual 1
Sealed bags 1
Note:
1.This package insert must be read in its entirety before using this product.
2.This ELISA Kit is used for lab research only!
"(本产品仅供体外科研用途,不可用于临床诊断!)
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文献和实验20万元转让DNA抽提系列试剂盒 本试剂盒是由复旦大学博士后研制。考虑到目前国内大多数实验室研究经费的实际情况,我们力求研制出性价比比较高的试剂盒。配方、生产工艺、生产材料经过一系列优化,操作方便、生产设备简单、生产材料价廉。生产成本是目前国内最低的。用该系列试剂盒分离、纯化的DNA纯度不亚于国外同类试剂盒,而且DNA的得率较高。经过三年的销售使用,客户反映该系列试剂盒质量可靠、价格低廉,是目前市场上性价比最高的DNA抽提系列试剂盒。该试剂盒使用率非常高。深受用户欢迎。 本系列试剂盒
Incorporation of Genetically Modified Cells in Chicken Chimeras
is limited by the inability to routinely and efficiently produce transgenic birds. One proposed strategy toward accessing the avian genome is the use of transgenic chimeric intermediates. As described in Chapter 37, dispersed chicken blastodermal cells (CBCs
蛋白质,如聚赖氨酸、鱼精蛋白等作预包被,也可提高核酸的结合力。也可用亲和素生物素系统作间接包被,即用亲和素先包被载体,然后加入生物素化的DNA,这种包被方法均匀、牢固,已扩大应用于各种抗原物质的定量测定。 脂类物质无法与固相载体结合,可将其在有机溶剂(例如乙醇)中溶解后加入ELISA板孔中,开盖置冰箱过夜或冷风吹干,待酒精挥发后,让脂质自然干固在固相表面。抗心磷脂抗体的ELISA试剂一般采用这种包被方式。 三、ELISA试剂盒的包被条件与封闭 包被用抗原或抗体的浓度,包被的温度和时间
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