- ￥3480
- LSM Bio
- 0.78-50ng/ml
- 0.469ng/ml
- 进口
- ELI-05096b
- 2025年07月15日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
100
- 供应商：
LSM BIO
- 检测范围：
0.78-50ng/ml
- 检测方法：
夹心法
- 应用：
利用ELISA方法体外定量检测牛血清，血浆等样本中目标蛋白的浓度
- 适应物种：
牛
- 标记物：
Bovine DNA nucleotidylexotransferase,DNTT
- 样本：
牛的血浆，血清，细胞上清等
- 灵敏度：
0.469ng/ml
- 规格：
96tests
Bovine DNA nucleotidylexotransferase, DNTT ELISA KIT
Product Name:Bovine DNA nucleotidylexotransferase, DNTT ELISA KIT
Packing:96T
Catalog No.:ELI-05096b
Gene Name:Bovine DNA nucleotidylexotransferase, DNTT
Detect Range:15.625-1000pg/ml
Sensitivity:9.375pg/ml
Target Protein Name:Bovine DNA nucleotidylexotransferase, DNTT
Alternative Name:DNTT,Bovine DNA nucleotidylexotransferase, DNTT
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Bovine DNA nucleotidylexotransferase, DNTT ELISA KIT allows for the in vitro quantitative determination of Bovine DNA nucleotidylexotransferase, DNTT concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Bovine DNA nucleotidylexotransferase, DNTT ELISA KIT has been pre-coated with an Bovine DNA nucleotidylexotransferase, DNTT antibody specific to Bovine DNA nucleotidylexotransferase, DNTT .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Bovine DNA nucleotidylexotransferase, DNTT and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Bovine DNA nucleotidylexotransferase, DNTT, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Bovine DNA nucleotidylexotransferase, DNTT in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验Production of Monoclonal Antibody by DNA Immunization with Electroporation
DNA immunization with in vivo electroporation is an efficient alternative protocol for the production of monoclonal antibodies (mAb). Generation of mAb by DNA immunization is a novel approach to circumvent the following technical hurdles
有替代前者的趋势。由于ABS-ELISA较普通ELISA多用了两种试剂，增加了操作步骤，在临床检验中ABS-ELISA应用不多。科研项目中检测微量的成分如细胞因子常采用本法。晶美分装ELISA KIT采用的方法：1， TORCH及传染病试剂盒（间接法），见2.2.32， TORCH-IgM捕获法特色：包被抗体，标记抗原原理：3， 细胞因子试剂盒采用的方法路线（ABC-ELISA）原理产品特色：采用ABC法，灵敏度更高，特异性更强。生物素抗体和酶联物是浓缩的，使用前需用相应的缓冲液稀释。酶联物可以通用
8.8 40mM MgCl2 160 mM NH4 (SO4)2 100 mM b -Mercaptoethanol 1 mg/ml bovine serum albumin. Tris-EDTA (TE) pH 8.0 DNA precipitation reagents: Phenol/Chloroform pH 8-9 3M NaOAc (for general precipitation) 5M NH4 Oac
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