- ￥3480
- LSM BIO
- 0.78-50ng/ml
- 0.469ng/ml
- 进口
- CELI-66096c
- 2025年07月13日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
100
- 供应商：
LSM BIO
- 检测范围：
0.78-50ng/ml
- 检测方法：
夹心法
- 应用：
利用ELISA方法体外定量检测鸡血清，血浆等样本中目标蛋白的浓度
- 适应物种：
鸡
- 标记物：
Chicken 17-Hydroxyprogesterone,17OHP
- 样本：
鸡的血浆，血清，细胞上清等
- 灵敏度：
0.469ng/ml
- 规格：
96tests
Chicken 17- Hydroxyprogesterone, 17- OHP ELISA Kit
96 Tests
Operating instructions
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE
BEGINNING!
Synonyms
17-Hydroxyprogesterone17-OH progesterone,17-OHP
Search name
Chicken 17-Hydroxyprogesterone ELISA Kit, Chicken 17-OHP ELISA Kit,17-Hydroxyprogesterone ELISA Kit,17-OHP ELISA Kit,
ELISA Kit, 17-Hydroxyprogesterone17-OH progesterone,17-OHP
Intended use
This immunoassay kit allows for the in vitro quantitative determination of general 17-hydroxyprogesterone,17-Hydroxyprogesterone
concentrations in serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids.
Test principle
The ELISA is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been
pre-coated with an antibody specific to 17-Hydroxyprogesterone, During the reaction, 17-Hydroxyprogesterone in the sample or standard
competes with a fixed amount of biotin-labeled 17-Hydroxyprogesterone for sites on a pre-coated Monoclonal antibody specific to
17-Hydroxyprogesterone. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to
Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The
enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured
spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of 17-Hydroxyprogesterone in the samples is then
determined by comparing the O.D. of the samples to the standard curve.
"(本产品仅供体外科研用途，不可用于临床诊断!)
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说明书为准。 保存条件及有效期 纤维素 fitc流式ZNF286IHC、FACS、WB、E 1．试剂盒保存：；2-8℃。 2．有效期：6 个月 人一氧化氮(NO)酶联试剂盒说明书 2-甲氧乙基酯 fitc流式ZNF537IHC、FACS、WB、ELISA Rabbit polyclonal to ZNF537 antibody 乙二胺 fitc流式ZNF5IHC、FACS、WB、ELISA Rabbit
,and the cell pellets were frozen and stored at –80ºC. Lysis Lysis was accomplished with B-PER reagent supplemented with 1/50 vol 10-mg/ml chicken egg-white lysozyme (stored at –80ºC in aliquots),1/125 vol 5-mg/ml DNaseI (in 20 mM sodium acetate,pH adjusted
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