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- 详细信息
- 文献和实验
- 技术资料
- 库存:
100
- 供应商:
LSM BIO
- 检测范围:
0.78-50ng/ml
- 检测方法:
夹心法
- 应用:
利用ELISA方法体外定量检测鸡血清,血浆等样本中目标蛋白的浓度
- 适应物种:
鸡
- 标记物:
Chicken Prostaglandin D2,PGD2
- 样本:
鸡的血浆,血清,细胞上清等
- 灵敏度:
0.469ng/ml
- 规格:
96tests
Chicken Prostaglandin D2, PGD2 ELISA Kit
96 Tests
Operating instructions
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE
BEGINNING!
Synonyms
Prostaglandin D2,PGD2
Search name
Chicken Prostaglandin D2 ELISA Kit, Chicken PGD2 ELISA Kit,Prostaglandin D2 ELISA Kit,PGD2 ELISA Kit, ELISA Kit
Intended use
This immunoassay kit allows for the in vitro quantitative determination of prostaglandin d2,Prostaglandin D2 concentrations in
serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids.
Test principle
The ELISA is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been
pre-coated with an antibody specific to Prostaglandin D2, During the reaction, Prostaglandin D2 in the sample or standard competes with
a fixed amount of biotin-labeled Prostaglandin D2 for sites on a pre-coated Monoclonal antibody specific to Prostaglandin D2. Excess
conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is
added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is
terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450
nm ± 2 nm. The concentration of Prostaglandin D2 in the samples is then determined by comparing the O.D. of the samples to the
standard curve.
"(本产品仅供体外科研用途,不可用于临床诊断!)
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文献和实验上海西唐生物科技有限公司 021-55229872, 65333639 www.westang.com 大鼠前列腺素 D2(PGD2)ELISA 试剂盒 ( 用于血清、血浆、细胞培养上清液和尿液生物体液内 ) 原理 本实验采用双抗体夹心 ABC-ELISA 法。用抗大鼠 PGD2 单抗包被于酶标板上,标准品和样品中的 PGD2 与单抗结合,加入生物素化的抗大鼠 PGD2 ,形成免疫复合物连接在板上
上海西唐生物科技有限公司 021-55229872, 65333639 www.westang.com 人磷脂酶D2 (sPLD2)ELISA 试剂盒 ( 用于血清、血浆、细胞培养上清液和其它生物体液内 ) 原理 本实验采用双抗体夹心 ABC-ELISA 法。用抗人 sPLD2 单抗包被于酶标板上,标准品和样品中的 sPLD2与单抗结合,加入生物素化的抗人 sPLD2
2 Dimensional Gel Electrophoretic Analysis for Chicken Egg
with distilled H2O 18. The stopping solution: 3.65 g EDTA-Na2¡D2 H2O •M Make up to 250 with distilled H2O Procedure 1. The whole egg contents of the chicken egg is sonicated for 5 minutes. Purpose: To breakdown the membranous
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