- ￥3480
- LSM Bio
- 0.78-50ng/ml
- 0.469ng/ml
- 进口
- ELA-E1039b
- 2025年07月15日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
100
- 供应商：
LSM BIO
- 检测范围：
0.78-50ng/ml
- 检测方法：
夹心法
- 应用：
利用ELISA方法体外定量检测牛血清，血浆等样本中目标蛋白的浓度
- 适应物种：
牛
- 标记物：
Bovine Pulmonary surfactant associated protein D,PSPD
- 样本：
牛的血浆，血清，细胞上清等
- 灵敏度：
0.469ng/ml
- 规格：
96tests
Bovine Pulmonary surfactant associated protein D, PSPD ELISA Kit
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE
BEGINNING!
Synonyms
Pulmonary surfactant-associated protein D,PSP-D,SP-D,Collectin-7,Lung surfactant protein D,surfactant protein DCOLEC7, PSPD,
SFTP4, SP-D, collectin 7, collectin-7, lung surfactant protein D, pulmonary surfactant apoprotein, pulmonary surfactant-associated protein
D, surfactant pulmonary-associated protein D, surfactant-associated protein pulmonary 4, COLEC7,CP4
Search name
Bovine Pulmonary surfactant-associated protein D ELISA KIT ,Bovine PSP-D ELISA KIT ,Bovine SP-D ELISA KIT , Bovine PSPD
ELISA KIT, Bovine Collectin-7 ELISA KIT ,Bovine Lung surfactant protein D ELISA KIT ,Bovine surfactant protein DCOLEC7
ELISA KIT , Bovine SFTP4 ELISA KIT ,Bovine SP-D ELISA KIT ,Bovine collectin 7 ELISA KIT ,Bovine collectin-7 ELISA
KIT ,Bovine lung surfactant protein D ELISA KIT ,Bovine pulmonary surfactant apoprotein ELISA KIT ,Bovine pulmonary
surfactant-associated protein D ELISA KIT ,Bovine surfactant pulmonary-associated protein D ELISA KIT ,Bovine
surfactant-associated protein pulmonary 4 ELISA KIT,Bovine COLEC7 ELISA KIT,Bovine CP4 ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of bovine pulmonary surfactant-associated protein d,PSP-D
concentrations in serum, Plasma, Urine, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to PSP-D. Standards or samples are then added to
the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for PSP-D and Avidin conjugated to
Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well.
Only those wells that contain PSP-D, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The
enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured
spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of PSP-D in the samples is then determined by comparing
the O.D. of the samples to the standard curve.
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文献和实验Purification of Surfactant Protein D (SP-D) from Pooled Amniotic Fluid and Bronchoalveolar Lavage
Surfactant protein SP-D is a multimeric collagenous lectin, called collectin . SP-D is a multifunctional, pattern recognition innate immune molecule, which binds in a calcium dependent manner to an array of carbohydrates and lipids
Mapping Protein Distributions on Polytene Chromosomes by Immunostaining
solution (a small spoonful of nonfat dry milk in 40 mL PBS) Bovine serum albumin (BSA) 3,3''-diaminobenzidine tetrahydrochloride (DAB; Sigma D5637) Detergent (optional, see Step 2) Drosophila (see Steps 7-9) Entellan (EMD
Methods for the Detection of D-Amino-Acid Oxidase
al. (5 ) using bovine serum albumin as a standard. It was also determined using a Protein Assay Kit (Bio-Rad, Hercules, CA). D-Amino-acid oxidase activity is finally expressed as the amount of D-alanine oxidized per min per milligram of protein. Hog
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