- ￥3480
- LSM BIO
- 0.78-50ng/ml
- 0.469ng/ml
- 进口
- ELI-38120d
- 2025年07月15日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
100
- 供应商：
LSM BIO
- 检测范围：
0.78-50ng/ml
- 检测方法：
夹心法
- 应用：
利用ELISA方法体外定量检测狗血清，血浆等样本中目标蛋白的浓度
- 适应物种：
狗
- 标记物：
Canine NPC2 (Epididymal secretory protein E1) (CUSTOM)
- 样本：
狗的血浆，血清，细胞上清等
- 灵敏度：
0.469ng/ml
- 规格：
96tests
Canine NPC2 (Epididymal secretory protein E1) ELISA Kit (CUSTOM)
Product Name:Canine NPC2 (Epididymal secretory protein E1) ELISA Kit (CUSTOM)
Packing:96T
Catalog No.:ELI-38120d
Gene Name:Canine NPC2 (Epididymal secretory protein E1) (CUSTOM)
Detect Range:31.25-2000pg/ml
Sensitivity:18.75pg/ml
Target Protein Name:Canine NPC2 (Epididymal secretory protein E1) (CUSTOM)
Alternative Name:NPC2 (Epididymal secretory protein E1) (CUSTOM),NPC2 (Epididymal secretory protein E1) (CUSTOM)
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Canine NPC2 (Epididymal secretory protein E1) ELISA Kit (CUSTOM) allows for the in vitro quantitative determination of Canine NPC2 (Epididymal secretory protein E1) (CUSTOM) concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in
Canine NPC2 (Epididymal secretory protein E1) ELISA Kit
96 Tests
Operating instructions
FOR RESEARCH USE ONLY!
Alternative name
Canine NPC2 ELISA KIT ,Canine EDDM1 ELISA KIT ,Canine HE1 ELISA KIT ,Canine Niemann-Pick disease type C2 protein ELISA KIT ,Canine Niemann-Pick disease ELISA KIT ,Canine type C2 ELISA KIT ,Canine epididymal protein 1 ELISA KIT ,Canine canine epididymis-specific protein 1 ELISA KIT ,Canine tissue-specific secretory protein ELISA KIT ,Canine NPC intracellular cholesterol transporter 2 ELISA KIT
Intended use
Canine NPC2 ELISA kit allows for the in vitro quantitative determination of Canine Epididymal secretory protein E1 concentrations in serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids.
Test principle
Canine NPC2 ELISA kit is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to NPC2, During the reaction, NPC2 in the sample or standard competes with a fixed amount of biotin-labeled NPC2 for sites on a pre-coated Monoclonal antibody specific to NPC2. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of NPC2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NPC2 Related Products
> NPC2 ELISA KITS
Bovine NPC2 (Epididymal secretory protein E1) ELISA Kit (CUSTOM)
Canine NPC2 (Epididymal secretory protein E1) ELISA Kit (CUSTOM)
Human NPC2 (Epididymal secretory protein E1) ELISA Kit (CUSTOM)
Mouse NPC2 (Epididymal secretory protein E1) ELISA Kit (CUSTOM)
Porcine NPC2 (Epididymal secretory protein E1) ELISA Kit (CUSTOM)
> NPC2 Antibodies
Polyclonal antibody-Canine Epididymal secretory protein E1
has been pre-coated with an Canine NPC2 (Epididymal secretory protein E1) (CUSTOM) antibody specific to Canine NPC2 (Epididymal secretory protein E1) (CUSTOM) .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Canine NPC2 (Epididymal secretory protein E1) (CUSTOM) and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Canine NPC2 (Epididymal secretory protein E1) (CUSTOM), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Canine NPC2 (Epididymal secretory protein E1) (CUSTOM) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
"(本产品仅供体外科研用途，不可用于临床诊断!)
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文献和实验Group 2a of the Coronaviridae family contains human and animal pathogens that include mouse hepatitis virus, rat coronavirus, human respiratory coronaviruses OC43 and the recently identified HKU1 strain, a newly recognized canine respiratory
失误所 致。胶体金簇如果多的话可能会堵塞膜孔。如果成簇的原因是由于胶体金的疏水 作用力形成的，那么在金标液流动的过程中胶体金颗粒也会粘附在捕捉抗体上。 无论待测物是否存在，金标的抗体都可能会与捕捉抗体发生非特异性免疫反 应。虽然在 ELISA 技术中在一对抗体之间发生类似的反应可能不是经常发生的， 但是这种方法会使硝酸纤维素膜上的标记抗体与捕捉抗体非常接近，从而提高了 发生非特异性免疫反应的可能性。另外，特别是在使用多克隆抗体时，有可能会 发生与样品中其它待测物的非特异性的反应，这种非特异性反应的发生与否主要
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