- ￥3480
- LSM BIO
- 0.78-50ng/ml
- 0.469ng/ml
- 进口
- CELI-66087c
- 2025年07月15日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
100
- 供应商：
LSM BIO
- 检测范围：
0.78-50ng/ml
- 检测方法：
夹心法
- 应用：
利用ELISA方法体外定量检测鸡血清，血浆等样本中目标蛋白的浓度
- 适应物种：
鸡
- 标记物：
Chicken Keratan sulfate,KS
- 样本：
鸡的血浆，血清，细胞上清等
- 灵敏度：
0.469ng/ml
- 规格：
96tests
Chicken Keratan sulfate, KS ELISA Kit
96 Tests
Operating instructions
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE
BEGINNING!
Synonyms
Keratan sulfate, keratosulfate,KS
Search name
Chicken KS ELISA Kit, Chicken keratosulfate ELISA Kit, Chicken Keratan sulfate ELISA Kit
Intended use
This immunoassay kit allows for the in vitro quantitative determination of Chicken keratan sulfate,Keratan sulfate concentrations in serum,
plasma, tissue homogenates, cell culture supernates, and other biological fluids.
Test principle
The ELISA is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been
pre-coated with an antibody specific to Keratan sulfate, During the reaction, Keratan sulfate in the sample or standard competes with a
fixed amount of biotin-labeled Keratan sulfate for sites on a pre-coated Monoclonal antibody specific to Keratan sulfate. Excess conjugate
and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each
microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the
addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The
concentration of Keratan sulfate in the samples is then determined by comparing the O.D. of the samples to the standard curve
"(本产品仅供体外科研用途，不可用于临床诊断!)
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文献和实验又称硫酸角质，与蛋白质形成结合体存在于哺乳类的角膜、椎间板、软骨和动脉中，也就是以蛋白多糖（ proteoglgcan）形式存在的一种粘多糖。在多数情况下和硫酸软骨素共存，也有时两者只有一个蛋白质部分。在胎儿期软骨的蛋白多糖中几乎不含有硫酸角质素，但随着年龄增加其含量渐增，这可作为动物结缔组织伴随年龄发生变化的例证。如图所示，硫酸角质素是以 D-半乳糖和 N-乙酰葡糖胺 -6-硫酸形成的双糖作为主要重复单位，一部分半乳糖在 6位上被硫酸化或分枝成
C. Avoid light exposure during preparation and storage. ABTS Stop Solution: Combine 50 ml dimethylformamide (DMF; Pierce, Cat. No. 20672) with 50 ml ddH2 O Add 20 g sodium dodecyl sulfate TMB
Glycosaminoglycans (GAGs) are a class of biopolymers that include chondrotin sulfate, dermatan sulfate, keratan sulfate, hyaluronic acid, heparin, and heparan sulfate. The GAGs are linear polysaccharides that are microheterogeneous
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