Canine Steroid 21-hydroxylase,

Canine Steroid 21-hydroxylase, CYP21 ELISA KIT

Product Name:Canine Steroid 21-hydroxylase, CYP21 ELISA KIT
Packing:96T

Catalog No.:ELI-05125d

Gene Name:Canine Steroid 21-hydroxylase, CYP21

Detect Range:31.25-2000pg/ml

Sensitivity:18.75pg/ml

Target Protein Name:Canine Steroid 21-hydroxylase, CYP21

Alternative Name:Steroid 21-hydroxylase,Steroid 21-hydroxylase, CYP21
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Canine Steroid 21-hydroxylase, CYP21 ELISA KIT allows for the in vitro quantitative determination of Canine Steroid 21-hydroxylase, CYP21 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in

Canine Steroid 21- hydroxylase, Cyp21 ELISA Kit

96 Tests

Operating instruction

 

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

 

Synonyms

Steroid 21-hydroxylase,Cytochrome P450-C21B,Cytochrome P450-C21,Cytochrome P450 XXI,Cytochrome P450 21,21-OHase,Cytochrome P-450c21, 21 hydroxylase,CYP21,CA21H,CAH1,CPS1,CYP21B,P450c21B,21-OHase,cytochrome P450 XXI,cytochrome P450, subfamily XXIA (steroid 21-hydroxylase, congenital adrenal hyperplasia), polypeptide 2,cytochrome P450-C21B,steroid 21-hydroxylase,steroid 21-monooxygenase,cytochrome P450, family 21 subfamily , polypeptide 2

 

Search name

Canine Steroid 21-hydroxylase ELISA KIT ,Canine Cytochrome P450-C21B ELISA KIT ,Canine Cytochrome P450-C21 ELISA KIT ,Canine Cytochrome P450 XXI ELISA KIT ,Canine Cytochrome P450 21 ELISA KIT ,Canine 21-OHase ELISA KIT ,Canine Cytochrome P-450c21 ELISA KIT ,Canine  21 hydroxylase ELISA KIT ,Canine CYP21 ELISA KIT ,Canine CA21H ELISA KIT ,Canine CAH1 ELISA KIT ,Canine CPS1 ELISA KIT ,Canine CYP21B ELISA KIT ,Canine P450c21B ELISA KIT ,Canine 21-OHase ELISA KIT ,Canine cytochrome P450 XXI ELISA KIT ,Canine cytochrome P450 ELISA KIT ,Canine  subfamily XXIA (steroid 21-hydroxylase ELISA KIT ,Canine  congenital adrenal hyperplasia) ELISA KIT ,Canine  polypeptide 2 ELISA KIT ,Canine cytochrome P450-C21B ELISA KIT ,Canine steroid 21-hydroxylase ELISA KIT ,Canine steroid 21-monooxygenase ELISA KIT ,Canine cytochrome P450 ELISA KIT ,Canine  family 21 subfamily  ELISA KIT ,Canine  polypeptide 2 ELISA KIT

 

Intended use

This immunoassay kit allows for the in vitro quantitative determination of Canine Steroid 21-hydroxylase concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

 

Test principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to Steroid 21-hydroxylase. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Steroid 21-hydroxylase and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Steroid 21-hydroxylase, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Steroid 21-hydroxylase in the samples is then determined by comparing the O.D. of the samples to the standard curve.

has been pre-coated with an Canine Steroid 21-hydroxylase, CYP21 antibody specific to Canine Steroid 21-hydroxylase, CYP21 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Canine Steroid 21-hydroxylase, CYP21 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Canine Steroid 21-hydroxylase, CYP21, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Canine Steroid 21-hydroxylase, CYP21 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!

  "(本产品仅供体外科研用途，不可用于临床诊断!)