Bovine Steroid 21- hydroxylase

Bovine Steroid 21- hydroxylase, Cyp21 ELISA Kit

96 Tests

Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

Synonyms

Steroid 21-hydroxylase,Cytochrome P450-C21B,Cytochrome P450-C21,Cytochrome P450 XXI,Cytochrome P450 21,21-OHase,Cytochrome P-450c21, 21 hydroxylase,CYP21,CA21H,CAH1,CPS1,CYP21B,P450c21B,21-OHase,cytochrome P450 XXI,cytochrome P450, subfamily XXIA (steroid 21-hydroxylase, congenital adrenal hyperplasia), polypeptide 2,cytochrome P450-C21B,steroid 21-hydroxylase,steroid 21-monooxygenase,cytochrome P450, family 21 subfamily , polypeptide 2

Search name

Bovine Steroid 21-hydroxylase ELISA KIT ,Bovine Cytochrome P450-C21B ELISA KIT ,Bovine Cytochrome P450-C21 ELISA KIT ,Bovine Cytochrome P450 XXI ELISA KIT ,Bovine Cytochrome P450 21 ELISA KIT ,Bovine 21-OHase ELISA KIT ,Bovine Cytochrome P-450c21 ELISA KIT ,Bovine  21 hydroxylase ELISA KIT ,Bovine CYP21 ELISA KIT ,Bovine CA21H ELISA KIT ,Bovine CAH1 ELISA KIT ,Bovine CPS1 ELISA KIT ,Bovine CYP21B ELISA KIT ,Bovine P450c21B ELISA KIT ,Bovine 21-OHase ELISA KIT ,Bovine cytochrome P450 XXI ELISA KIT ,Bovine cytochrome P450 ELISA KIT ,Bovine  subfamily XXIA (steroid 21-hydroxylase ELISA KIT ,Bovine  congenital adrenal hyperplasia) ELISA KIT ,Bovine  polypeptide 2 ELISA KIT ,Bovine cytochrome P450-C21B ELISA KIT ,Bovine steroid 21-hydroxylase ELISA KIT ,Bovine steroid 21-monooxygenase ELISA KIT ,Bovine cytochrome P450 ELISA KIT ,Bovine  family 21 subfamily  ELISA KIT ,Bovine  polypeptide 2 ELISA KIT

Intended use

This immunoassay kit allows for the in vitro quantitative determination of Bovine Steroid 21-hydroxylase concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to Steroid 21-hydroxylase. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Steroid 21-hydroxylase and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Steroid 21-hydroxylase, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Steroid 21-hydroxylase in the samples is then determined by comparing the O.D. of the samples to the standard curve.