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- 详细信息
- 文献和实验
- 技术资料
- 库存:
100
- 供应商:
LSM BIO
- 检测范围:
0.78-50ng/ml
- 检测方法:
夹心法
- 应用:
利用ELISA方法体外定量检测狗血清,血浆等样本中目标蛋白的浓度
- 适应物种:
狗
- 标记物:
Canine Cytochrome P450 2E1,CYP2E1
- 样本:
狗的血浆,血清,细胞上清等
- 灵敏度:
0.469ng/ml
- 规格:
96tests
Canine Cytochrome P450 2E1, CYP2E1 ELISA KIT
Product Name:Canine Cytochrome P450 2E1, CYP2E1 ELISA KIT
Packing:96T
Catalog No.:ELI-03183d
Gene Name:Canine Cytochrome P450 2E1, CYP2E1
Detect Range:15.625-1000pg/ml
Sensitivity:9.375pg/ml
Target Protein Name:Canine Cytochrome P450 2E1, CYP2E1
Alternative Name:Cytochrome P450 2E1,Cytochrome P450 2E1, CYP2E1
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Canine Cytochrome P450 2E1, CYP2E1 ELISA KIT allows for the in vitro quantitative determination of Canine Cytochrome P450 2E1, CYP2E1 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle: Canine Cytochrome P450 2E1, Cyp2e1 ELISA Kit 96 Tests Operating instruction FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING! Synonyms Cytochrome P450 2E1,Cytochrome P450RLM6,Cytochrome P450-J,4-nitrophenol 2-hydroxylase,CYPIIE1, Cyp2e1,cytochrome P450 2E1; CPE1; CYP2E; P450-J; P450C2E; 4-nitrophenol 2-hydroxylase; CYPIIE1; cytochrome P450 subfamily IIE ethanol-inducible polypeptide 1; cytochrome P450-J; flavoprotein-linked monooxygenase; microsomal monooxygenase; xenobiotic monooxygenase; cytochrome P450 family 2 subfamily E polypeptide 1 Search name Canine Cytochrome P450 2E1 ELISA KIT ,Canine Cytochrome P450RLM6 ELISA KIT ,Canine Cytochrome P450-J ELISA KIT ,Canine 4-nitrophenol 2-hydroxylase ELISA KIT ,Canine CYPIIE1 ELISA KIT ,Canine Cyp2e1 ELISA KIT ,Canine cytochrome P450 2E1 ELISA KIT ,Canine CPE1 ELISA KIT ,Canine CYP2E ELISA KIT ,Canine P450-J ELISA KIT ,Canine P450C2E ELISA KIT ,Canine 4-nitrophenol 2-hydroxylase ELISA KIT ,Canine CYPIIE1 ELISA KIT ,Canine cytochrome P450 subfamily IIE ethanol-inducible polypeptide 1 ELISA KIT ,Canine cytochrome P450-J ELISA KIT ,Canine flavoprotein-linked monooxygenase ELISA KIT ,Canine microsomal monooxygenase ELISA KIT ,Canine xenobiotic monooxygenase ELISA KIT ,Canine cytochrome P450 family 2 subfamily E polypeptide 1 ELISA KIT Intended use This immunoassay kit allows for the in vitro quantitative determination of Canine Cytochrome P450 2E1 concentrations in serum, Plasma,, tissue homogenates and Cell culture supernates and Other biological fluids. Test principle The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cytochrome P450 2E1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Cytochrome P450 2E1 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Cytochrome P450 2E1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Cytochrome P450 2E1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
The microtiter plate provided in
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
"(本产品仅供体外科研用途,不可用于临床诊断!)
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文献和实验Genotyping for Cytochrome P450 Polymorphisms
Protocols for the extraction of DNA from human blood and for genotyping for a number of common cytochrome P450 polymorphisms using either polymerase chain reaction (PCR)-restriction fragment length polymorphism or PCR-single-strand
Cytochrome P450 Reconstitution Systems
Human liver microsomes contain multiple forms of cytochrome P450 (CYP or P450) that catalyze oxidation of a number of xenobiotic and endobiotic chemicals. Individual P450 forms have unique, but overlapping, substrate specificities
Evaluation of Cytochrome P450 Activities in Human Hepatocytes In Vitro
Major hepatic cytochrome P450 activities (CYP1A2, CYP2A6, CYP2B6, CYP2C9, CYP2C19, CYP2D6, CYP2E1, and CYP3A4) can be simultaneously examined in human hepatocytes by incubation with a cocktail of multiple specific probes. Cocktail strategy
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