- ￥3480
- LSM Bio
- 0.78-50ng/ml
- 0.469ng/ml
- 进口
- ELI-03182b
- 2025年07月13日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
100
- 供应商：
LSM BIO
- 检测范围：
0.78-50ng/ml
- 检测方法：
夹心法
- 应用：
利用ELISA方法体外定量检测牛血清，血浆等样本中目标蛋白的浓度
- 适应物种：
牛
- 标记物：
Bovine Cytochrome P450 2E1,CYP2E1
- 样本：
牛的血浆，血清，细胞上清等
- 灵敏度：
0.469ng/ml
- 规格：
96tests
Bovine Cytochrome P450 2E1, Cyp2e1 ELISA Kit
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
Cytochrome P450 2E1,Cytochrome P450RLM6,Cytochrome P450-J,4-nitrophenol 2-hydroxylase,CYPIIE1, Cyp2e1,cytochrome P450 2E1; CPE1; CYP2E; P450-J; P450C2E; 4-nitrophenol 2-hydroxylase; CYPIIE1; cytochrome P450 subfamily IIE ethanol-inducible polypeptide 1; cytochrome P450-J; flavoprotein-linked monooxygenase; microsomal monooxygenase; xenobiotic monooxygenase; cytochrome P450 family 2 subfamily E polypeptide 1
Search name
Bovine Cytochrome P450 2E1 ELISA KIT ,Bovine Cytochrome P450RLM6 ELISA KIT ,Bovine Cytochrome P450-J ELISA KIT ,Bovine 4-nitrophenol 2-hydroxylase ELISA KIT ,Bovine CYPIIE1 ELISA KIT ,Bovine Cyp2e1 ELISA KIT ,Bovine cytochrome P450 2E1 ELISA KIT ,Bovine CPE1 ELISA KIT ,Bovine CYP2E ELISA KIT ,Bovine P450-J ELISA KIT ,Bovine P450C2E ELISA KIT ,Bovine 4-nitrophenol 2-hydroxylase ELISA KIT ,Bovine CYPIIE1 ELISA KIT ,Bovine cytochrome P450 subfamily IIE ethanol-inducible polypeptide 1 ELISA KIT ,Bovine cytochrome P450-J ELISA KIT ,Bovine flavoprotein-linked monooxygenase ELISA KIT ,Bovine microsomal monooxygenase ELISA KIT ,Bovine xenobiotic monooxygenase ELISA KIT ,Bovine cytochrome P450 family 2 subfamily E polypeptide 1 ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of Bovine Cytochrome P450 2E1 concentrations in serum, Plasma,, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cytochrome P450 2E1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Cytochrome P450 2E1 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Cytochrome P450 2E1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Cytochrome P450 2E1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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文献和实验Genotyping for Cytochrome P450 Polymorphisms
Protocols for the extraction of DNA from human blood and for genotyping for a number of common cytochrome P450 polymorphisms using either polymerase chain reaction (PCR)-restriction fragment length polymorphism or PCR-single-strand
Evaluation of Cytochrome P450 Activities in Human Hepatocytes In Vitro
Major hepatic cytochrome P450 activities (CYP1A2, CYP2A6, CYP2B6, CYP2C9, CYP2C19, CYP2D6, CYP2E1, and CYP3A4) can be simultaneously examined in human hepatocytes by incubation with a cocktail of multiple specific probes. Cocktail strategy
Development and Properties of HepG2 Cells That Constitutively Express CYP2E1
CYP2E1, a member of the cytochrome P450 family, is induced by ethanol. CYP2E1 activates many hepatotoxins to their reactive toxic intermediate form, and generates reactive oxygen species (ROS) during its catalytic cycle. Induction of CYP2E1
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