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- 详细信息
- 文献和实验
- 技术资料
- 库存:
100
- 供应商:
LSM BIO
- 检测范围:
0.78-50ng/ml
- 检测方法:
夹心法
- 应用:
利用ELISA方法体外定量检测狗血清,血浆等样本中目标蛋白的浓度
- 适应物种:
狗
- 标记物:
Canine Triglyceride,TG
- 样本:
狗的血浆,血清,细胞上清等
- 灵敏度:
0.469ng/ml
- 规格:
96tests
Canine Triglyceride, TAG ELISA Kit
96 Tests
Operating instructions
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
Triglyceride, TG,triacylglycerol,TAG,triacylglyceride
Search name
Canine Triglyceride ELISA KIT ,Canine TG ELISA KIT ,Canine triacylglycerol ELISA KIT ,Canine TAG ELISA KIT ,Canine triacylglyceride ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of Canine Triglyceride concentrations in serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids.
Test principle
The ELISA is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Triglyceride, During the reaction, Triglyceride in the sample or standard competes with a fixed amount of biotin-labeled Triglyceride for sites on a pre-coated Monoclonal antibody specific to Triglyceride. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Triglyceride in the samples is then determined by comparing the O.D. of the samples to the standard curve.
"(本产品仅供体外科研用途,不可用于临床诊断!)
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文献和实验充分摇匀后,室温放置5-10分钟,3500转/分钟,吸弃上清液,测各沉淀管的放射性计数(p)。 四.结果分析 1.参考值:TG抗体B/T≥30%为阳性。 2.临床意义:可用于自身免疫疾病的诊断。 五注意事项 1收到试剂盒后,尽快存放在2-8°C冰箱中。 2阴性对照管在数据处理时非常重要,要做复管。 3分离剂加前一定要摇匀。 4加样时应避免血清样品间的交叉污染。 5加样前试剂要平衡到室温并摇匀。 六质量控制 1每批测定都使用北京
大鼠甘油三酯(TG )酶联免疫分析 试剂盒使用说明书 本试剂仅供研究使用 目的:本试剂盒用于测定大鼠血清,血浆,细胞上清及相关液体样本中甘油三酯(TG )的含量。 实验原理: 本试剂盒应用双抗体夹心法测定标本中大鼠甘油三酯( TG )水平。用纯化的大鼠甘油三酯( TG )抗体包被微孔板,制成固相抗体,往包被单抗的微孔中依次加入甘油三酯( TG ),再与 HRP 标记的甘油三酯( TG )抗体结合,形成抗体
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