- ￥3480
- LSM Bio
- 0.78-50ng/ml
- 0.469ng/ml
- 进口
- ELI-52354b
- 2025年07月16日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
100
- 供应商：
LSM BIO
- 检测范围：
0.78-50ng/ml
- 检测方法：
夹心法
- 应用：
利用ELISA方法体外定量检测牛血清，血浆等样本中目标蛋白的浓度
- 适应物种：
牛
- 标记物：
Bovine Alpha-soluble NSF attachment protein,NAPA
- 样本：
牛的血浆，血清，细胞上清等
- 灵敏度：
0.469ng/ml
- 规格：
96tests
Bovine Alpha- soluble NSF attachment protein, NAPA ELISA KIT
Product Name:Bovine Alpha- soluble NSF attachment protein, NAPA ELISA KIT
Packing:96T
Catalog No.:ELI-52354b
Gene Name:Bovine Alpha-soluble NSF attachment protein, NAPA
Detect Range:31.2-2000pg/ml
Sensitivity:18.75pg/ml
Target Protein Name:Bovine Alpha-soluble NSF attachment protein, NAPA
Alternative Name:NAPA,Bovine Alpha-soluble NSF attachment protein, NAPA
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Bovine Alpha- soluble NSF attachment protein, NAPA ELISA KIT allows for the in vitro quantitative determination of Bovine Alpha-soluble NSF attachment protein, NAPA concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Bovine Alpha- soluble NSF attachment protein, NAPA ELISA KIT has been pre-coated with an Bovine Alpha-soluble NSF attachment protein, NAPA antibody specific to Bovine Alpha-soluble NSF attachment protein, NAPA .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Bovine Alpha-soluble NSF attachment protein, NAPA and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Bovine Alpha-soluble NSF attachment protein, NAPA, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Bovine Alpha-soluble NSF attachment protein, NAPA in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验Considerations for useThe Bradford assay is very fast and uses about the same amount of protein as the Lowry assay.It is fairly accurate and samples that are out of range can be retested within minutes.The Bradford is recommended for general use
. The Bradford assay is rather sensitive to bovine serum albumin, more so than "average" proteins, by about a factor of two. Immunoglogin G (IgG - gamma globulin) is the preferred protein standard. The addition of 1 M NaOH was suggested by Stoscheck (1990
Studies of Secretion Using Permeabilized Platelets
that are required for the platelet-release reaction. Recent advances by several groups have elucidated at least some of the proteins required for platelet exocytosis. Soluble NSF attachment protein receptor (SNARE) proteins mediate platelet granule-plasma membrane
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