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- 详细信息
- 文献和实验
- 技术资料
- 库存:
100
- 供应商:
LSM BIO
- 检测范围:
0.78-50ng/ml
- 检测方法:
夹心法
- 应用:
利用ELISA方法体外定量检测牛血清,血浆等样本中目标蛋白的浓度
- 适应物种:
牛
- 标记物:
Bovine Carbonic anhydrase 2,CA2
- 样本:
牛的血浆,血清,细胞上清等
- 灵敏度:
0.469ng/ml
- 规格:
96tests
Bovine carbonic anhydrase 2, CA- 2 ELISA Kit
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE
BEGINNING!
Synonyms
Carbonic anhydrase 2,; Carbonate dehydratase II,Carbonic anhydrase II(CA-II),; Car2; AI131712; CAII; Ca2; Car-2; Ltw-5; Lvtw-5; CA II;
CA-II;
Search name
Bovine carbonic anhydrase 2 ELISA Kit, Bovine CA-2 ELISA Kit, Bovine Carbonate dehydratase II ELISA Kit, Bovine CA-II
ELISA Kit, Bovine Car2 ELISA Kit, Bovine AI131712 ELISA Kit, Bovine Car-2 ELISA Kit, Bovine Ltw-5 ELISA Kit, Bovine
CA2 ELISA Kit, Bovine Lvtw-5 ELISA Kit, Bovine CA II ELISA Kit,
Intended use
This immunoassay kit allows for the in vitro quantitative determination of Bovine Carbonic anhydrase 2 concentrations in serum, Plasma,
tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to Carbonic anhydrase 2. Standards or samples are
then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Carbonic
anhydrase 2 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB
substrate solution is added to each well. Only those wells that contain Carbonic anhydrase 2, biotin-conjugated antibody and
enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid
solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Carbonic
anhydrase 2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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文献和实验Analysis of Proteins using Small Format 2D Gel Electrophoresis
(obtained from Pharmacia Biotech): bovine serum albumin (67 kDa), ovalbumin (43 kDa), carbonic anhydrase (30 kDa) and trypsin inhibitor (20.1 kDa). Post-electrophoretic identification of proteins Various protocols
Analysis of Proteins using Small Format 2D Gel Electrophoresis
with the following proteins of known molecular weights (obtained from Pharmacia Biotech): bovine serum albumin (67 kDa), ovalbumin (43 kDa), carbonic anhydrase (30 kDa) and trypsin inhibitor (20.1 kDa). Post-electrophoretic identification of proteins
【资源】全面归类网址:毕赤酵母表达各种来源蛋白(细菌,真菌,植物,人类等)
Porcine follicle-stimulating hormoneS, 10 mg/L, PHO1[174]Porcine inhibitor of carbonic anhydrase (transferrin family)S, 5 mg/L, α-MF[175]Porcine single-chain interleukin-12S, 4 mg/L,α-MF, active370Porcine leukocyte 12-lipoxygenaseI[176]Porcine lactoferrinS
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