- ￥3480
- LSM Bio
- 0.78-50ng/ml
- 0.469ng/ml
- 进口
- ELI-50595b
- 2025年07月16日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
100
- 供应商：
LSM BIO
- 检测范围：
0.78-50ng/ml
- 检测方法：
夹心法
- 应用：
利用ELISA方法体外定量检测牛血清，血浆等样本中目标蛋白的浓度
- 适应物种：
牛
- 标记物：
Bovine cAMP-responsive element modulator,CREM
- 样本：
牛的血浆，血清，细胞上清等
- 灵敏度：
0.469ng/ml
- 规格：
96tests
Bovine cAMP- responsive element modulator, CREM ELISA KIT
Product Name:Bovine cAMP- responsive element modulator, CREM ELISA KIT
Packing:96T
Catalog No.:ELI-50595b
Gene Name:Bovine cAMP-responsive element modulator, CREM
Detect Range:0.156-10ng/ml
Sensitivity:0.094ng/ml
Target Protein Name:Bovine cAMP-responsive element modulator, CREM
Alternative Name:CREM,Bovine cAMP-responsive element modulator, CREM
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Bovine cAMP- responsive element modulator, CREM ELISA KIT allows for the in vitro quantitative determination of Bovine cAMP-responsive element modulator, CREM concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Bovine cAMP- responsive element modulator, CREM ELISA KIT has been pre-coated with an Bovine cAMP-responsive element modulator, CREM antibody specific to Bovine cAMP-responsive element modulator, CREM .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Bovine cAMP-responsive element modulator, CREM and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Bovine cAMP-responsive element modulator, CREM, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Bovine cAMP-responsive element modulator, CREM in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验Pasteur, Paris, France Excerpted from Protein: Protein Interactions, Second Edition Edited by Erica A. Golemis and Peter D. Adams ABSTRACT cAMP measurements are obtained using an ELISA assay (Harlow
dangyonghui2 大侠们，我现在用的是promega公司的cAMP测定试剂盒，按照说明书上面要求做的标准曲线，可是我做了几次出来和说明书上面都不一样，是怎么回事？求解！！！ 现在有测cAMP的同志们一起讨论下，我的Q:1186130200 bianbenjamin 我们用的是PE的盒子 LANCETM cAMP 384Kit（PE公司） 1.检测原理：
【求助】如何提取细胞中的cAMP？和普通蛋白的裂解buffer一样么？
试剂盒出售，不是基于ELISA法的，比ELISA法更方便。 苏叶 我用的是125I的RIA kit，我们核同位素室的老师说，照常处理你的细胞，离心去上清后加入0.4ml 1M的高氯酸就可以了。不过要预实验一下，摸索浓度。 我这几天也在做细胞内cAMP和cGMP检测。 sosorunning 普通的Lysis buffer 就可以，但是你得加IBMX抑制PDE的活性。不知道你是想做western还是干吗？ 如果你是要测
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