- ￥3480
- LSM Bio
- 0.78-50ng/ml
- 0.469ng/ml
- 进口
- ELI-49179b
- 2025年07月12日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
100
- 供应商：
LSM BIO
- 检测范围：
0.78-50ng/ml
- 检测方法：
夹心法
- 应用：
利用ELISA方法体外定量检测牛血清，血浆等样本中目标蛋白的浓度
- 适应物种：
牛
- 标记物：
Bovine Annexin A9,ANXA9
- 样本：
牛的血浆，血清，细胞上清等
- 灵敏度：
0.469ng/ml
- 规格：
96tests
Bovine Annexin A9, ANXA9 ELISA KIT
Product Name:Bovine Annexin A9, ANXA9 ELISA KIT
Packing:96T
Catalog No.:ELI-49179b
Gene Name:Bovine Annexin A9, ANXA9
Detect Range:31.2-2000pg/ml
Sensitivity:18.75pg/ml
Target Protein Name:Bovine Annexin A9, ANXA9
Alternative Name:ANXA9,Bovine Annexin A9, ANXA9
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Bovine Annexin A9, ANXA9 ELISA KIT allows for the in vitro quantitative determination of Bovine Annexin A9, ANXA9 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Bovine Annexin A9, ANXA9 ELISA KIT has been pre-coated with an Bovine Annexin A9, ANXA9 antibody specific to Bovine Annexin A9, ANXA9 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Bovine Annexin A9, ANXA9 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Bovine Annexin A9, ANXA9, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Bovine Annexin A9, ANXA9 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验Guide to Cell Proliferation and Apoptosis Methods
Annexin-V-FLUOS 32 Annexin-V-FLUOS Staining Kit 32 Annexin-V-AlexaTM 568 32 Annexin-V-Biotin 34
酶的活性。Intergen公司的TRAP-eze Telemerase Detection Kit在1996年率先推出。它提供特定的寡核苷酸底物，分别与底物及端粒重复序列配对的引物。如果待测样本中含有端粒酶活性，就能在底物上接上不同个数的6碱基(GGTTAG)端粒重复序列，通过PCR反应，产物电泳检测就可观察到相差六个碱基的DNA Ladder现象(参见图4)。此外，Intergen公司还提供用酶联免疫法(ELISA)检测的试剂盒. 同样，这种检测方法也不专对细胞凋亡，检测结果也不纯反应细胞凋亡的发生
性的磷脂结合蛋白，能专一性的结合暴露在膜外侧的PS，再通过简单的显色或发光系统进行检测。由于这是一种凋亡早期的活细胞检测（悬浮细胞和贴壁细胞都适用），可与DNA染料或别的晚期检测方法相结合来标记凋亡的发展阶段。 美国著名生物试剂公司CLONTECH和INTERGEN公司分别开发了多种标记的Annexin V产品，简便快速，10分钟就可完成检测。其中带荧光标记的Annexin V-EGFP（Enhanced Green Fluorescent Protein）及Annexin V-FITC，灵敏
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