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从原料到标记,打造属于中国自己的流式抗体品牌Cytoscape 教程来了!快速实现顶刊同款通路网络图五大应用案例:Mustang Q 膜层析应用全解析1 个小工具,一次性搞定流程图、质粒图谱和信号通路图- 详细信息
- 文献和实验
- 技术资料
- 库存:
100
- 供应商:
LSM BIO
- 检测范围:
0.78-50ng/ml
- 检测方法:
夹心法
- 应用:
利用ELISA方法体外定量检测牛血清,血浆等样本中目标蛋白的浓度
- 适应物种:
牛
- 标记物:
Bovine Obg-like ATPase 1,OLA1
- 样本:
牛的血浆,血清,细胞上清等
- 灵敏度:
0.469ng/ml
- 规格:
96tests
Bovine Obg- like ATPase 1, OLA1 ELISA KIT
Product Name:Bovine Obg- like ATPase 1, OLA1 ELISA KIT
Packing:96T
Catalog No.:ELI-44590b
Gene Name:Bovine Obg-like ATPase 1, OLA1
Detect Range:0.313-20ng/ml
Sensitivity:0.188ng/ml
Target Protein Name:Bovine Obg-like ATPase 1, OLA1
Alternative Name:OLA1,Bovine Obg-like ATPase 1, OLA1
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Bovine Obg- like ATPase 1, OLA1 ELISA KIT allows for the in vitro quantitative determination of Bovine Obg-like ATPase 1, OLA1 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Bovine Obg- like ATPase 1, OLA1 ELISA KIT has been pre-coated with an Bovine Obg-like ATPase 1, OLA1 antibody specific to Bovine Obg-like ATPase 1, OLA1 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Bovine Obg-like ATPase 1, OLA1 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Bovine Obg-like ATPase 1, OLA1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Bovine Obg-like ATPase 1, OLA1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验,是国内科研人员查询和获取标准化细胞资源的重要平台。 ATCC ATCC 是国际上最具权威性的生物资源保藏机构之一,提供经过严格鉴定的细胞系及其详细培养条件说明,广泛作为国际科研和期刊投稿中推荐的细胞来源参考。 产品推荐 特级胎牛血清,乌拉圭 www.medchemexpress.cn/inhibitor-kit/fetal-bovine-serum
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mitochondrial transition pore formation by binding cyclophilin D. The Image-iT LIVE Mitochondrial Transition Pore Assay Kit has been tested with HeLa cells and bovine pulmonary artery endothelial cells (BPAEC). Each Image-iT LIVE Mitochondrial Transition Pore
。 重复性高:柱式法消除了手动匀浆带来的人为误差,只要离心力和时间固定,实验结果批间差小。 组分清晰,一盒多用:一次实验不仅能拿到质膜蛋白,还能同时获得胞浆蛋白、细胞器蛋白和核蛋白(粗提),方便你进行亚细胞定位分析。 兼容性广:获取的质膜沉淀可以用于纳米材料修饰,脂质组学分析。溶解沉淀后获得的蛋白可用于SDS-PAGE、Western Blot、ELISA、IP、Co-IP、质谱(MS)、2-D电泳等多种下游应用。 操作有多简单?真的就是“过柱子” 以细胞样品为例
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