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- 详细信息
- 文献和实验
- 技术资料
- 库存:
100
- 供应商:
LSM BIO
- 检测范围:
0.78-50ng/ml
- 检测方法:
夹心法
- 应用:
利用ELISA方法体外定量检测牛血清,血浆等样本中目标蛋白的浓度
- 适应物种:
牛
- 标记物:
Bovine Dipeptidyl aminopeptidase-like protein 6,DPP6
- 样本:
牛的血浆,血清,细胞上清等
- 灵敏度:
0.469ng/ml
- 规格:
96tests
Bovine Dipeptidyl aminopeptidase-like protein 6, DPP6 ELISA Kit
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
Dipeptidyl aminopeptidase-like protein 6,DPP6,Dipeptidyl peptidase VI,DPP VI,Dipeptidyl peptidase IV-like protein,Dipeptidyl peptidase 6,DPPX,Dipeptidyl aminopeptidase-related protein, DPPX; VF2; dipeptidyl aminopeptidase IV-related protein; dipeptidyl aminopeptidase-like protein 6; dipeptidyl peptidase IV-related protein; dipeptidyl peptidase VI; dipeptidylpeptidase 6; dipeptidyl-peptidase 6
Search name
Bovine Dipeptidyl aminopeptidase-like protein 6 ELISA KIT ,Bovine DPP6 ELISA KIT ,Bovine Dipeptidyl peptidase VI ELISA KIT ,Bovine DPP VI ELISA KIT ,Bovine Dipeptidyl peptidase IV-like protein ELISA KIT ,Bovine Dipeptidyl peptidase 6 ELISA KIT ,Bovine DPPX ELISA KIT ,Bovine Dipeptidyl aminopeptidase-related protein ELISA KIT ,Bovine DPPX ELISA KIT ,Bovine VF2 ELISA KIT ,Bovine dipeptidyl aminopeptidase IV-related protein ELISA KIT ,Bovine dipeptidyl peptidase IV-related protein ELISA KIT ,Bovine dipeptidyl peptidase VI ELISA KIT ,Bovine dipeptidylpeptidase 6 ELISA KIT ,Bovine dipeptidyl-peptidase 6 ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of Bovine dipeptidyl aminopeptidase-like protein 6,DPP6 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to DPP6. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for DPP6 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain DPP6, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of DPP6 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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文献和实验【资源】全面归类网址:毕赤酵母表达各种来源蛋白(细菌,真菌,植物,人类等)
[151,152,153, 387]Bovine lysozyme c2S, 550 mg/L, native[147]Bovine mannose 6-phosphate receptorsS, α-MF, 28 mg purify. protein/L517Bovine opsinS*, 0.3 mg/L, PHO1[148]Bovine pancreatic trypsin inhibitor (aprotinin)S, 930 mg/L, α-MF[149]Bovine tissue-type
Analysis of Oxidative Modification of Proteins
6.8 ( appendix 2A ) Bicinchoninic acid protein assay kit (BCA; Pierce Co.) Bovine serum albumin (BSA
High‐Throughput, Multiplexed Analysis of 3‐Nitrotyrosine in Individual Proteins
Daly, D.S., Anderson, K.K., White, A.M., Gonzalez, R.M., Varnum, S.M., and Zangar, R.C. 2008. Predicting protein concentrations with ELISA microarray assays, monotonic splines and Monte Carlo simulation. Stat. Appl. Genet. Mol. Biol. 7: Article21
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