- ￥3480
- LSM Bio
- 0.78-50ng/ml
- 0.469ng/ml
- 进口
- ELI-45701b
- 2025年07月15日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
100
- 供应商：
LSM BIO
- 检测范围：
0.78-50ng/ml
- 检测方法：
夹心法
- 应用：
利用ELISA方法体外定量检测牛血清，血浆等样本中目标蛋白的浓度
- 适应物种：
牛
- 标记物：
Bovine M-phase inducer phosphatase 1,CDC25A
- 样本：
牛的血浆，血清，细胞上清等
- 灵敏度：
0.469ng/ml
- 规格：
96tests
Bovine M- phase inducer phosphatase 1, CDC25A ELISA KIT
Product Name:Bovine M- phase inducer phosphatase 1, CDC25A ELISA KIT
Packing:96T
Catalog No.:ELI-45701b
Gene Name:Bovine M-phase inducer phosphatase 1, CDC25A
Detect Range:31.2-2000pg/ml
Sensitivity:18.75pg/ml
Target Protein Name:Bovine M-phase inducer phosphatase 1, CDC25A
Alternative Name:CDC25A,Bovine M-phase inducer phosphatase 1, CDC25A
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Bovine M- phase inducer phosphatase 1, CDC25A ELISA KIT allows for the in vitro quantitative determination of Bovine M-phase inducer phosphatase 1, CDC25A concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Bovine M- phase inducer phosphatase 1, CDC25A ELISA KIT has been pre-coated with an Bovine M-phase inducer phosphatase 1, CDC25A antibody specific to Bovine M-phase inducer phosphatase 1, CDC25A .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Bovine M-phase inducer phosphatase 1, CDC25A and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Bovine M-phase inducer phosphatase 1, CDC25A, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Bovine M-phase inducer phosphatase 1, CDC25A in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验thousands of colored products. Thus, in ELISA, antigens are detectable when present in only nanogram and picogram quantities. One form of the ELISA is carried out in a microtiter plate which is used as the solid phase for adsorption of antigen (Figure
DETECTION OF RECOMBINANT PROTEINS BY ELISA
them together overnight at 4°C, or for 2 hours at 37°C. Rinse plates 5 times with PBS-Tween. Add 50 µl per well of the alkaline phosphatase-conjugated secondary antibody diluted 1:2000 in ELISA conjugate diluent. Incubate for 2 hours at room temperature
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