- ￥3480
- LSM Bio
- 0.78-50ng/ml
- 0.469ng/ml
- 进口
- ELI-45847b
- 2025年07月15日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
100
- 供应商：
LSM BIO
- 检测范围：
0.78-50ng/ml
- 检测方法：
夹心法
- 应用：
利用ELISA方法体外定量检测牛血清，血浆等样本中目标蛋白的浓度
- 适应物种：
牛
- 标记物：
Bovine LIM domain kinase 2,LIMK2
- 样本：
牛的血浆，血清，细胞上清等
- 灵敏度：
0.469ng/ml
- 规格：
96tests
Bovine LIM domain kinase 2, LIMK2 ELISA KIT
Product Name:Bovine LIM domain kinase 2, LIMK2 ELISA KIT
Packing:96T
Catalog No.:ELI-45847b
Gene Name:Bovine LIM domain kinase 2, LIMK2
Detect Range:78-5000pg/ml
Sensitivity:46.9pg/ml
Target Protein Name:Bovine LIM domain kinase 2, LIMK2
Alternative Name:LIMK2,Bovine LIM domain kinase 2, LIMK2
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Bovine LIM domain kinase 2, LIMK2 ELISA KIT allows for the in vitro quantitative determination of Bovine LIM domain kinase 2, LIMK2 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Bovine LIM domain kinase 2, LIMK2 ELISA KIT has been pre-coated with an Bovine LIM domain kinase 2, LIMK2 antibody specific to Bovine LIM domain kinase 2, LIMK2 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Bovine LIM domain kinase 2, LIMK2 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Bovine LIM domain kinase 2, LIMK2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Bovine LIM domain kinase 2, LIMK2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验Using Phospho‐Motif Antibodies to Determine Kinase Substrates
.C., Asara, J.M., Lane, W.S., Garner, C.C., and Lienhard, G.E. 2002. A method to identify serine kinase substrates. Akt phosphorylates a novel adipocyte protein with a Rab GTPase‐activating protein (GAP) domain. J. Biol. Chem. 277:22115‐22118
systems usually only require a single protein,called Cas9, to perform the target cleavage. Recent years, thetype II CRISPR-Cas9 systems have been studied by several groupsin their native microbial domain to elucidate their molecular organizationsand
Antigen Design Sera Purification Tech Sheet
domain then the choice is simple. For example, if one is studying proteolytic processing of an N-terminal precursor, antibodies against the N-terminal region of interest would be raised. Likewise if the goal is to monitor the phosphorylation state
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