- ￥3480
- LSM Bio
- 0.78-50ng/ml
- 0.469ng/ml
- 进口
- ELI-43741b
- 2026年05月21日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
100
- 供应商：
LSM BIO
- 检测范围：
0.78-50ng/ml
- 检测方法：
夹心法
- 应用：
利用ELISA方法体外定量检测牛血清，血浆等样本中目标蛋白的浓度
- 适应物种：
牛
- 标记物：
Bovine Leukocyte elastase inhibitor,SERPINB1
- 样本：
牛的血浆，血清，细胞上清等
- 灵敏度：
0.469ng/ml
- 规格：
96tests
Bovine Leukocyte elastase inhibitor, SERPINB1 ELISA KIT
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
Leukocyte elastase inhibitor, Serpin B1,Monocyte/neutrophil elastase inhibitor,Peptidase inhibitor 2,SERPINB1,ELANH2;EI,HEL57; LEI; M/NEI; MNEI; PI-2; PI2; epididymis luminal protein 57; leukocyte elastase inhibitor; peptidase inhibitor 2; protease inhibitor 2 (anti-elastase), monocyte/neutrophil derived; serine proteinase inhibitor clade B member 1; serpin peptidase inhibitor clade B member 1
Search name
Bovine Leukocyte elastase inhibitor ELISA KIT ,Bovine Serpin B1 ELISA KIT ,Bovine Monocyte/neutrophil elastase inhibitor ELISA KIT ,Bovine Peptidase inhibitor 2 ELISA KIT ,Bovine SERPINB1 ELISA KIT ,Bovine ELANH2 ELISA KIT ,Bovine EI ELISA KIT ,Bovine HEL57 ELISA KIT ,Bovine LEI ELISA KIT ,Bovine M/NEI ELISA KIT ,Bovine MNEI ELISA KIT ,Bovine PI-2 ELISA KIT ,Bovine PI2 ELISA KIT ,Bovine epididymis luminal protein 57 ELISA KIT ,Bovine leukocyte elastase inhibitor ELISA KIT ,Bovine peptidase inhibitor 2 ELISA KIT ,Bovine protease inhibitor 2 (anti-elastase) ELISA KIT ,Bovine monocyte/neutrophil derived ELISA KIT ,Bovine serine proteinase inhibitor clade B member 1 ELISA KIT ,Bovine serpin peptidase inhibitor clade B member 1 ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of Bovine leukocyte elastase inhibitor,LEI concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to LEI. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for LEI and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain LEI, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of LEI in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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文献和实验，是国内科研人员查询和获取标准化细胞资源的重要平台。 ATCC ATCC 是国际上最具权威性的生物资源保藏机构之一，提供经过严格鉴定的细胞系及其详细培养条件说明，广泛作为国际科研和期刊投稿中推荐的细胞来源参考。 产品推荐 特级胎牛血清，乌拉圭 www.medchemexpress.cn/inhibitor-kit/fetal-bovine-serum
【资源】全面归类网址：毕赤酵母表达各种来源蛋白（细菌，真菌，植物，人类等）
[151,152,153, 387]Bovine lysozyme c2S, 550 mg/L, native[147]Bovine mannose 6-phosphate receptorsS, α-MF, 28 mg purify. protein/L517Bovine opsinS*, 0.3 mg/L, PHO1[148]Bovine pancreatic trypsin inhibitor (aprotinin)S, 930 mg/L, α-MF[149]Bovine tissue-type
Immunohistochemistry: Fluorescence Protocol 2.0
Peroxidase Blocking Buffer 3M NaN3 in EBSS with 1% H2 O2 and 0.1% (w/v) Saponin. Endogenous Biotin Blocking Buffer Avidin/Biotin Blocking Kit (Vector Lab Catalog # SP2001). Both Avidin D and Biotin should be supplemented with 0.1% (w/v) Saponin
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