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- 详细信息
- 文献和实验
- 技术资料
- 库存:
100
- 供应商:
LSM BIO
- 检测范围:
0.78-50ng/ml
- 检测方法:
夹心法
- 应用:
利用ELISA方法体外定量检测牛血清,血浆等样本中目标蛋白的浓度
- 适应物种:
牛
- 标记物:
Bovine Matrix metalloproteinase-20,MMP20
- 样本:
牛的血浆,血清,细胞上清等
- 灵敏度:
0.469ng/ml
- 规格:
96tests
Bovine Matrix metalloproteinase- 20, MMP20 ELISA KIT
Product Name:Bovine Matrix metalloproteinase- 20, MMP20 ELISA KIT
Packing:96T
Catalog No.:ELI-42772b
Gene Name:MMP20
Detect Range:3.12-200 ng/mL
Sensitivity:0.39 ng/mL
Target Protein Name:Bovine Matrix metalloproteinase- 20
Alternative Name:MMP20,Bovine Matrix metalloproteinase- 20
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Bovine Matrix metalloproteinase- 20, MMP20 ELISA KIT allows for the in vitro quantitative determination ofBovine Matrix metalloproteinase- 20 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Bovine Matrix metalloproteinase- 20, MMP20 ELISA KIT has been pre-coated with an MMP20 antibody specific to Bovine Matrix metalloproteinase- 20 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Bovine Matrix metalloproteinase- 20 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Bovine Matrix metalloproteinase- 20, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Bovine Matrix metalloproteinase- 20 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验were eluted with two 3-ml portions of elution buffer (Pierce kit); diluted to 13 ml with TBS; dialyzed at 4ºC against two changes of TBS and three changes of PBS (0.15 M NaCl,5 mM NaH2PO4,pH adjusted to 7.0 with NaOH); and stored at –20ºC.Protein concentration
for labeling buffer (Cat. No. BP328-1), 25-ml pipets for make-shift size-exclusion chromatography columns (Cat. No. 13-674-41E), and Pasteur pipettes (Cat. No. 13-678-20D) for filling the columns were from Fisher. Sephadex G-50 resin (Cat. No. G-50-150
v468 Chapter 9 自身启动子报告基因验证转录靶点
, S., Hlubek, F.,Kirchner, T. (1999) beta-catenin regulates the expression of the matrix metalloproteinase-7 in human colorectal cancer. Amer J Pathol 155 , 1033–1038. 7. Crawford, H.C., Fingleton, B.M., Rudolph-Owen, L.A., Goss, K.J., Rubinfeld
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