- ￥3480
- LSM Bio
- 0.78-50ng/ml
- 0.469ng/ml
- 进口
- ELI-42763b
- 2025年07月16日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
100
- 供应商：
LSM BIO
- 检测范围：
0.78-50ng/ml
- 检测方法：
夹心法
- 应用：
利用ELISA方法体外定量检测牛血清，血浆等样本中目标蛋白的浓度
- 适应物种：
牛
- 标记物：
Bovine cGMP-dependent protein kinase 1,PRKG1
- 样本：
牛的血浆，血清，细胞上清等
- 灵敏度：
0.469ng/ml
- 规格：
96tests
Bovine cGMP- dependent protein kinase 1, PRKG1 ELISA KIT
Product Name:Bovine cGMP- dependent protein kinase 1, PRKG1 ELISA KIT
Packing:96T
Catalog No.:ELI-42763b
Gene Name:Bovine cGMP-dependent protein kinase 1, PRKG1
Detect Range:78-5000pg/ml
Sensitivity:46.9pg/ml
Target Protein Name:Bovine cGMP-dependent protein kinase 1, PRKG1
Alternative Name:PRKG1,Bovine cGMP-dependent protein kinase 1, PRKG1
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Bovine cGMP- dependent protein kinase 1, PRKG1 ELISA KIT allows for the in vitro quantitative determination of Bovine cGMP-dependent protein kinase 1, PRKG1 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Bovine cGMP- dependent protein kinase 1, PRKG1 ELISA KIT has been pre-coated with an Bovine cGMP-dependent protein kinase 1, PRKG1 antibody specific to Bovine cGMP-dependent protein kinase 1, PRKG1 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Bovine cGMP-dependent protein kinase 1, PRKG1 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Bovine cGMP-dependent protein kinase 1, PRKG1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Bovine cGMP-dependent protein kinase 1, PRKG1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验Purification and Activation of Cyclic GMP-Dependent Protein Kinase
Cyclic GMP (cGMP)-dependent protein kinase (G kinase) can be purified from bovine lung with the combination of ion-exchange chromatography, ammonium sulfate precipitation, and cyclic nucleotide affinity chromatography (1 ). In this chapter
Using Phospho‐Motif Antibodies to Determine Kinase Substrates
of substrates by protein kinases regulates a myriad of cellular processes, ranging from proliferation and migration to autophagy, senescence, and apoptosis. Kinase substrate selectivity is largely dependent on the amino acid sequence surrounding
A Novel Fluorescent Transcriptional Reporter for Cell-Based Microarray Assays
for gain-of-function mutations in the type-I cGMP-dependent protein kinase (PRKG1). We have identified a constitutively active mutant of this enzyme in which a conserved Glu at position 81 was mutated to Lys.
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