- ￥3480
- LSM Bio
- 0.78-50ng/ml
- 0.469ng/ml
- 进口
- ELI-22459b
- 2025年07月10日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
100
- 供应商：
LSM BIO
- 检测范围：
0.78-50ng/ml
- 检测方法：
夹心法
- 应用：
利用ELISA方法体外定量检测牛血清，血浆等样本中目标蛋白的浓度
- 适应物种：
牛
- 标记物：
Bovine V-type proton ATPase subunit D,ATP6V1D
- 样本：
牛的血浆，血清，细胞上清等
- 灵敏度：
0.469ng/ml
- 规格：
96tests
Bovine V- type proton ATPase subunit D, ATP6V1D ELISA KIT
Product Name:Bovine V- type proton ATPase subunit D, ATP6V1D ELISA KIT
Packing:96T
Catalog No.:ELI-22459b
Gene Name:Bovine V-type proton ATPase subunit D, ATP6V1D
Detect Range:31.2-2000pg/ml
Sensitivity:18.75pg/ml
Target Protein Name:Bovine V-type proton ATPase subunit D, ATP6V1D
Alternative Name:ATP6V1D,Bovine V-type proton ATPase subunit D, ATP6V1D
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Bovine V- type proton ATPase subunit D, ATP6V1D ELISA KIT allows for the in vitro quantitative determination of Bovine V-type proton ATPase subunit D, ATP6V1D concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Bovine V- type proton ATPase subunit D, ATP6V1D ELISA KIT has been pre-coated with an Bovine V-type proton ATPase subunit D, ATP6V1D antibody specific to Bovine V-type proton ATPase subunit D, ATP6V1D .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Bovine V-type proton ATPase subunit D, ATP6V1D and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Bovine V-type proton ATPase subunit D, ATP6V1D, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Bovine V-type proton ATPase subunit D, ATP6V1D in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验). 3. Tris-buffered saline (TBS) with Tween (TBS-T): Prepare10× stock with 1.37 M NaCl, 27 mM KCl, 250 mM Tris-HCl, pH 7.4, and 1% (v/v) Tween-20. 4. Blocking buffer: 4% (w/v) bovine serum albumin (BSA) and0.1% (w/v) sodium azide in TBS. 5. Primary
Determination of Muscle Fiber Type in Rodents
, Inc. Keywords: SDH; ATPase; myosin; oxidative enzymes; muscle fiber type GO TO THE FULL PROTOCOL: PDF or HTML at Wiley Online Library
V294.PART II，Chapter 5 细胞粘附实验（英文版）
. Cines, D. B., Pollak, E. S., Buck, C. A., Loscalzo, J., Zimmerman, G. A., McEver,R. P., et al. (1998) Endothelial cells in physiology and in the pathophysiology ofvascular disorders. Blood 91, 3527–3561. 13. Lawrence, M. B., McIntire, L. V., and Eskin
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