- ￥3480
- LSM Bio
- 0.78-50ng/ml
- 0.469ng/ml
- 进口
- ELI-40959b
- 2025年07月14日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
100
- 供应商：
LSM BIO
- 检测范围：
0.78-50ng/ml
- 检测方法：
夹心法
- 应用：
利用ELISA方法体外定量检测牛血清，血浆等样本中目标蛋白的浓度
- 适应物种：
牛
- 标记物：
Bovine Spindlin-2,SPIN2
- 样本：
牛的血浆，血清，细胞上清等
- 灵敏度：
0.469ng/ml
- 规格：
96tests
Bovine Spindlin- 2, SPIN2 ELISA KIT
Product Name:Bovine Spindlin- 2, SPIN2 ELISA KIT
Packing:96T
Catalog No.:ELI-40959b
Gene Name:Bovine Spindlin-2, SPIN2
Detect Range:0.156-10ng/ml
Sensitivity:0.094ng/ml
Target Protein Name:Bovine Spindlin-2, SPIN2
Alternative Name:SPIN2,Bovine Spindlin-2, SPIN2
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Bovine Spindlin- 2, SPIN2 ELISA KIT allows for the in vitro quantitative determination of Bovine Spindlin-2, SPIN2 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Bovine Spindlin- 2, SPIN2 ELISA KIT has been pre-coated with an Bovine Spindlin-2, SPIN2 antibody specific to Bovine Spindlin-2, SPIN2 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Bovine Spindlin-2, SPIN2 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Bovine Spindlin-2, SPIN2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Bovine Spindlin-2, SPIN2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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除>95%的蛋白提取和样品制备过程中所使用的去垢剂，而蛋白和多肽损失极少。此树脂适用于常用去垢剂的去除，如浓度在0.5-1%的SDS、Triton X-100、NP-40和CHAPS。目前有预装的离心柱和离心板形式，最多可处理100 µL样品。另外，您也可以购买HiPPR Detergent Removal Spin Column Kit，其中包含树脂浆和空的离心柱，便于您自己调整样品体积。 整个处理过程相当简单，仅需15分钟。首先是离心去除保存液，之后用缓冲液平衡树脂并离心，重复
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