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- 详细信息
- 技术资料
- 保存条件：
-2-8℃
- 保质期：
1年
- 英文名：
Bovine IgA ELISA Kit
- 库存：
大量
- 供应商：
上海钰博生物科技有限公司
- 规格：
盒
Bovine IgA ELISA Kit
Bovine IgA ELISA Kit are a highly sensitive two-site enzyme linked immunoassay (ELISA) for measuring IgA in bovine biological samples. In this assay the IgA present in samples reacts with the anti-IgA antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, anti-IgA antibodies conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled antibodies form complexes with the previously bound IgA. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3,5,5-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of IgA in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of IgA in the test sample. The quantity of IgA in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.
Product Description
Bovine IgA ELISA Kit
Bovine IgA ELISA Kit are a highly sensitive two-site enzyme linked immunoassay (ELISA) for measuring IgA in bovine biological samples. In this assay the IgA present in samples reacts with the anti-IgA antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, anti-IgA antibodies conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled antibodies form complexes with the previously bound IgA. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3,5,5-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of IgA in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of IgA in the test sample. The quantity of IgA in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.
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Bovine IgA ELISA Kit
$495
