- ￥3480
- LSM Bio
- 0.78-50ng/ml
- 0.469ng/ml
- 进口
- ELI-40392b
- 2025年07月14日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
100
- 供应商：
LSM BIO
- 检测范围：
0.78-50ng/ml
- 检测方法：
夹心法
- 应用：
利用ELISA方法体外定量检测牛血清，血浆等样本中目标蛋白的浓度
- 适应物种：
牛
- 标记物：
Bovine NEDD8-conjugating enzyme UBE2F,UBE2F
- 样本：
牛的血浆，血清，细胞上清等
- 灵敏度：
0.469ng/ml
- 规格：
96tests
Bovine NEDD8- conjugating enzyme UBE2F, UBE2F ELISA KIT
Product Name:Bovine NEDD8- conjugating enzyme UBE2F, UBE2F ELISA KIT
Packing:96T
Catalog No.:ELI-40392b
Gene Name:Bovine NEDD8-conjugating enzyme UBE2F, UBE2F
Detect Range:15.625-1000pg/ml
Sensitivity:9.375pg/ml
Target Protein Name:Bovine NEDD8-conjugating enzyme UBE2F, UBE2F
Alternative Name:UBE2F,Bovine NEDD8-conjugating enzyme UBE2F, UBE2F
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Bovine NEDD8- conjugating enzyme UBE2F, UBE2F ELISA KIT allows for the in vitro quantitative determination of Bovine NEDD8-conjugating enzyme UBE2F, UBE2F concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Bovine NEDD8- conjugating enzyme UBE2F, UBE2F ELISA KIT has been pre-coated with an Bovine NEDD8-conjugating enzyme UBE2F, UBE2F antibody specific to Bovine NEDD8-conjugating enzyme UBE2F, UBE2F .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Bovine NEDD8-conjugating enzyme UBE2F, UBE2F and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Bovine NEDD8-conjugating enzyme UBE2F, UBE2F, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Bovine NEDD8-conjugating enzyme UBE2F, UBE2F in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验Studies of the Ubiquitin Proteasome System
both in vitro and in cells. Because another protein modifier, NEDD8, itself regulates aspects of the ubiquitin system, basic protocols on neddylation are also included in this unit. Keywords: Ubiquitin; ubiquitylation; proteasomal degradation; protein
Analysis of Oxidative Modification of Proteins
6.8 ( appendix 2A ) Bicinchoninic acid protein assay kit (BCA; Pierce Co.) Bovine serum albumin (BSA
High‐Throughput, Multiplexed Analysis of 3‐Nitrotyrosine in Individual Proteins
. In addition, modifications in single?tissue?associated proteins released into blood can provide insight into the tissue localization of the inflammation or oxidative stress. We have developed an enzyme?linked immunosorbent assay antibody microarray platform
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