- ￥3480
- LSM BIO
- 0.78-50ng/ml
- 0.469ng/ml
- 进口
- ELI-40861d
- 2025年07月05日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
100
- 供应商：
LSM BIO
- 检测范围：
0.78-50ng/ml
- 检测方法：
夹心法
- 应用：
利用ELISA方法体外定量检测狗血清，血浆等样本中目标蛋白的浓度
- 适应物种：
狗
- 标记物：
Canine SEC61G
- 样本：
狗的血浆，血清，细胞上清等
- 灵敏度：
0.469ng/ml
- 规格：
96tests
Canine SEC61G ELISA KIT
Product Name:Canine SEC61G ELISA KIT
Packing:96T
Catalog No.:ELI-40861d
Gene Name:Canine SEC61G
Detect Range:0.313-20ng/ml
Sensitivity:0.188ng/ml
Target Protein Name:Canine SEC61G
Alternative Name:SEC61G,SEC61G
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Canine SEC61G ELISA KIT allows for the in vitro quantitative determination of Canine SEC61G concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Canine SEC61G ELISA KIT has been pre-coated with an Canine SEC61G antibody specific to Canine SEC61G .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Canine SEC61G and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Canine SEC61G, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Canine SEC61G in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
"(本产品仅供体外科研用途，不可用于临床诊断!)
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文献和实验>>> 分装 ELISA 试剂盒 F28100 狗脂联素 (Canine adiponectin) ELISA 48T/96T 2000/3000 进口分装 F28110 狗 C
4℃预冷的Buffer R-A，迅速用力碾磨至Buffer R-A 完全融化，继续碾磨30sec。 （4）将匀浆转入1.5 ml 离心管。如匀浆残留较多，加100μl Buffer R-A，适当研磨后，收集合并到1.5ml 离心管。 （5）用带4～6 号针头的1ml 注射器反复吸注10次。 匀浆充分与否是决定RNA 和DNA 得率的关键。匀浆完全时，溶液不再粘稠，很容易从注射器针头上呈不连续滴状滴落，而非连续状。 [酵母菌] （1）取5×107对数生长期酵母菌，10000×g 离心20
Modification Reagent and 3 M NaOH stock must be freshly prepared prior to each use. b. Sodium bisulfite modifies only single-stranded DNA. Double stranded DNA should be denatured in 200 mM NaOH prior to modification. Some highly G/C rich sequences
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