- ￥3480
- LSM Bio
- 0.78-50ng/ml
- 0.469ng/ml
- 进口
- ELI-40092b
- 2025年07月14日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
100
- 供应商：
LSM BIO
- 检测范围：
0.78-50ng/ml
- 检测方法：
夹心法
- 应用：
利用ELISA方法体外定量检测牛血清，血浆等样本中目标蛋白的浓度
- 适应物种：
牛
- 标记物：
Bovine TPST2 (Protein-tyrosine sulfotransferase 2) (C
- 样本：
牛的血浆，血清，细胞上清等
- 灵敏度：
0.469ng/ml
- 规格：
96tests
Bovine TPST2 (Protein- tyrosine sulfotransferase 2) ELISA Kit (CUSTOM)
96 Tests
Operating instructions
FOR RESEARCH USE ONLY!
Alternative name
Bovine Tpst2 ELISA KIT ,Bovine TANGO13B ELISA KIT ,Bovine TPST-2 ELISA KIT ,Bovine transport and golgi organization 13 homolog B ELISA KIT ,Bovine tyrosylprotein phosphotransferase 2 ELISA KIT ,Bovine tyrosylprotein sulfotransferase-2 ELISA KIT
Intended use
Bovine TPST2 ELISA kit allows for the in vitro quantitative determination of Bovine Protein- tyrosine sulfotransferase 2 concentrations in serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids.
Test principle
Bovine TPST2 ELISA kit is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to TPST2, During the reaction, TPST2 in the sample or standard competes with a fixed amount of biotin-labeled TPST2 for sites on a pre-coated Monoclonal antibody specific to TPST2. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of TPST2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Customized product Min.Order 5kits
Delivery time 4-6month
Order Bovine TPST2 ELISA kit by email:sales@
@
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文献和实验were eluted with two 3-ml portions of elution buffer (Pierce kit); diluted to 13 ml with TBS; dialyzed at 4ºC against two changes of TBS and three changes of PBS (0.15 M NaCl,5 mM NaH2PO4,pH adjusted to 7.0 with NaOH); and stored at –20ºC.Protein concentration
Analysis of Oxidative Modification of Proteins
6.8 ( appendix 2A ) Bicinchoninic acid protein assay kit (BCA; Pierce Co.) Bovine serum albumin (BSA
所有PKC亚型了，那为什么还要分磷酸化不同的残基呢？ 谢谢！ zhidanguilai 加拿大Immunechem公司的非放射性蛋白激酶C活性分析试剂盒安全、快速、稳定，可用于筛选蛋白激酶C抑制剂或激活剂、定性定量分析纯化或部分纯化的PKC激酶活性。 测试盒原理： 基于ELISA的基本原理。PKC底物被包被在酶标板上，在ATP存在的前提下，PKC激酶将底物磷酸化，洗掉游离的PKC激酶和其他成分。再加入特异性磷酸化抗体（只识别
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