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- 详细信息
- 文献和实验
- 技术资料
- 英文名:
ReadiUse™ 1 Kb Plus DNA Ladder
- 库存:
50
- 供应商:
广州市左克生物科技发展有限公司
- 规格:
100ul
| Ex (nm) | - | Em (nm) | - |
| 分子量 | - | 溶剂 | Water |
| 存储条件 | 在零下15度以下保存, 避免光照 |
ReadiUse 1 kb Plus DNA Ladder是即用型DNA Ladder。 它由26个单独的DNA片段组成:25k,10k,8k,6k,5k,4k,3k,2.5k,2k,1.5k,1.2k,1k,900、800、700、600、500、450、400, 350、300、250、200、150、100和50个碱基对,它们来自PCR产物和特异性消化的质粒DNA的混合物。 该产品包含四个增强的频段(3k,1.2k,500和200 bp),以方便参考。 此外,还添加了三种示踪染料,分别模拟电泳过程中4,000 bp,500 bp和50 bp dsDNA迁移的二甲苯基氰基FF,溴酚蓝和橙G,以进行实时检测。
参考文献
The pPSU Plasmids for Generating DNA Molecular Weight Markers.
Authors: Henrici, Ryan C and Pecen, Turner J and Johnston, James L and Tan, Song
Journal: Scientific reports (2017): 2438
Capillary electrophoresis combining three-step multiplex polymerase chain reactions for diagnosing α-thalassemia.
Authors: Chen, Yen-Ling
Journal: Electrophoresis (2011): 379-85
Antitumor activity of decoy oligodeoxynucleotides targeted to NF-kappaB in vitro and in vivo.
Authors: Wang, Tao and Li, Qing-Hua and Hao, Gang-Ping and Zhai, Jing
Journal: Asian Pacific journal of cancer prevention : APJCP (2010): 193-200
Actinobacillus actinomycetemcomitans induces apoptosis in human monocytic THP-1 cells.
Authors: Kato, Satsuki and Sugimura, Norihiko and Nakashima, Keisuke and Nishihara, Tatsuji and Kowashi, Yusuke
Journal: Journal of medical microbiology (2005): 293-298
Luteal phase serum cell-free DNA as a marker of failed pregnancy after assisted reproductive technology.
Authors: Hart, Elaine A and Patton, William C and Jacobson, John D and King, Alan and Corselli, Johannah and Chan, Philip J
Journal: Journal of assisted reproduction and genetics (2005): 213-7
[Apoptosis of human carcinoma of mouth floor KB cells and multidrug resistant KBv200 cells induced by azide methyl anthraquinone derivative].
Authors: Ding, Yan and He, Li-rong and Cao, Ka-jia and Lu, Yu and Gu, Lian-quan and Fu, Li-wu
Journal: Yao xue xue bao = Acta pharmaceutica Sinica (2005): 22-6
Evidence that DNA fragmentation in apoptosis is initiated and propagated by single-strand breaks.
Authors: Walker, P R and LeBlanc, J and Sikorska, M
Journal: Cell death and differentiation (1997): 506-15
Cytotoxicity and DNA damage associated with pyrazoloacridine in MCF-7 breast cancer cells.
Authors: Grem, J L and Politi, P M and Berg, S L and Benchekroun, N M and Patel, M and Balis, F M and Sinha, B K and Dahut, W and Allegra, C J
Journal: Biochemical pharmacology (1996): 1649-59
The evaluation and implementation of match criteria for forensic analysis of DNA.
Authors: Laber, T L and Iverson, J T and Liberty, J A and Giese, S A
Journal: Journal of forensic sciences (1995): 1058-64
Pulsed field capillary electrophoresis of multikilobase length nucleic acids in dilute methyl cellulose solutions.
Authors: Kim, Y and Morris, M D
Journal: Analytical chemistry (1994): 3081-5
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文献和实验. :: Electrophoresis buffer can affect the resolution of DNA. TAE (Tris-Acetate-EDTA) buffer provides better resolution of fragments >4 kb, while TBE (Tris-Borate-EDTA) buffer provides better resolution
A safe method of extracting DNA from Coccidioides immitis
: strain 1; c, d: strain 2; a, c: without Proteinase K; b, d: with Proteinase K; L: 1 kb DNA size ladder (Gibco BRL). Gel was treated with RNAse after electrophoresis.
first, which minimizes foaming during heating.Electrophoresis buffer can affect the resolution of DNA. TAE (Tris-Acetate-EDTA) buffer provides better resolution of fragments >4 kb, while TBE (Tris-Borate-EDTA) buffer provides better resolution of 0.1- to 3-kb fragments
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