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- 详细信息
- 技术资料
- 英文名：
Xite™ Red beta-D-galactopyranoside
- 库存：
50
- 供应商：
广州市左克生物科技发展有限公司
- 规格：
1mg
| Ex (nm) | - | Em (nm) | - |
| 分子量 | 591.63 | 溶剂 | DMSO |
| 存储条件 | 在零下15度以下保存, 避免光照 |
Xite Red β-D-半乳糖吡喃糖苷是β-半乳糖苷酶（β-gal）的荧光底物。与现有的β-半乳糖苷酶底物（例如，常用的FDG）相比，它具有更好的细胞通透性。Xite Red β-D-吡喃半乳糖苷很容易进入细胞，并被β-gal裂解，从而产生强荧光产品Xite Red。强烈荧光的Xite Red可以很好地保留在细胞中，从而易于通过流式细胞仪和荧光显微镜进行检测。Xite Red β-D-半乳糖吡喃糖苷提供了一种简单而灵敏的工具来检测β-半乳糖苷酶的活性。Xite Red β-D-吡喃半乳糖苷可以作为检测细胞中细胞衰老的工具，因为β-gal已被确定为细胞衰老的可靠标记。此外，Xite Red β-D-吡喃半乳糖苷是可固定的。Xite Red β-D-吡喃半乳糖苷产生的红色荧光可以很容易地与其他颜色的荧光探针（例如DAPI或GFP）结合使用，以进行多色荧光分析。，为您提供优质的Xite Red β-D-半乳糖吡喃糖苷。
适用仪器
| 荧光显微镜 | |
| Ex: | Cy3/TRITC 滤波片组 |
| Em: | Cy3/TRITC 滤波片组 |
| 推荐孔板: | 黑色透明底板 |
| 流式细胞仪 | |
| Ex: | 488激光 |
| Em: | 575/26 nm 滤波片 |
| 通道: | PE通道 |
样品实验方案
以下是我们推荐的方案，仅提供指导。具体实验应根据您的特定需求进行修改。
简要概述
- 根据需要处理样品
- 准备Xite Red β-D-吡喃半乳糖苷工作溶液并将其添加到样品中
- 在37°C下孵育样品15至45分钟
- 使用带有575/26 nm滤光片的流式细胞仪（PE通道）或带有Cy3/TRITC滤光片组的荧光显微镜检测荧光强度
溶液配制
储备溶液配制
操作步骤
- 根据需要处理样品。
- 处理并用自备的缓冲液（例如DPBS）洗涤细胞。
- 加入Xite Red β-D-吡喃半乳糖苷工作溶液15-45分钟，然后在37°C的培养箱中培养样品。
注意：孵育的佳时间需要通过实验确定。 - 取出工作溶液并用自备的缓冲液洗涤细胞。
- 将细胞重悬在自备的缓冲液中，并使用流式细胞仪使用575/26 nm滤光片（PE通道）或带有Cy3/TRITC滤光片组的荧光显微镜检测荧光强度。
图示
 图1.用Xite Red β-D-吡喃半乳糖苷测量β-gal的表达。将9L-LacZ细胞（过度表达β-gal的细胞）与Xite Red β-D-吡喃半乳糖苷在37°C下孵育30分钟。使用NovoCyte流式细胞仪（ACEA Biosciences）通过PE通道获取信号。 |
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