ReadiUse 细菌细胞裂解液*5X*

样品实验方案 

溶液配制 

ReadiUse 细菌细胞裂解缓冲液工作液（1X）： 将1 mL ReadiUse 细菌细胞裂解缓冲液（5X）加入4 mL  Mili-Q水中，并充分混合。

 

样品示例及操作

图1.细胞裂解是指细胞的分解，通常用于分析特定的细胞组成，例如蛋白质，脂质，核酸，报告分子，细胞信号分子和其他小生物分子。根据所用的裂解液，全部或部分膜被溶解。ReadiUse 试剂需要少的动手时间。这种细菌细胞裂解缓冲液只需简单的5倍稀释即可。它广泛用于裂解细胞以定量细菌中的小生物分子，例如NAD（P）/ NAD（P）H。

 

参考文献

Application of lysis system in bacterial vector vaccines
Authors: Tang, Y., Liu, Q., Li, P., Luo, H., Kong, Q.
Journal: Sheng Wu Gong Cheng Xue Bao (2019): 375-388

Bacteriophage Therapy Increases Complement-Mediated Lysis of Bacteria and Enhances Bacterial Clearance After Acute Lung Infection With Multidrug-Resistant Pseudomonas aeruginosa
Authors: Abd El-Aziz, A. M., Elgaml, A., Ali, Y. M.
Journal: J Infect Dis (2019): 1439-1447

FRET probe-based antibacterial susceptibility testing (F-AST) by detection of bacterial nucleases released by antibiotic-induced lysis
Authors: Park, K., Jeong, J., Yi, S. Y., Lee, W. S., Shin, Y. B.
Journal: Biosens Bioelectron (2019): 225-229

Mechanics and Dynamics of Bacterial Cell Lysis
Authors: Wong, F., Amir, A.
Journal: Biophys J (2019): 2378-2389

Phage single-gene lysis: Finding the weak spot in the bacterial cell wall
Authors: Chamakura, K., Young, R.
Journal: J Biol Chem (2019): 3350-3358

Quantifying bacterial cell lysis using GFP based fluorimetric assay
Authors: Sharma, M., Tyagi, J. L., Poluri, K. M.
Journal: Int J Biol Macromol (2019): 881-889

Use of a modified bacterial ghost lysis system for the construction of an inactivated avian pathogenic Escherichia coli vaccine candidate
Authors: Hu, J., Zuo, J., Chen, Z., Fu, L., Lv, X., Hu, S., Shi, X., Jing, Y., Wang, Y., Wang, Z., Mi, R., Huang, Y., Liu, D., Qi, K., Han, X.
Journal: Vet Microbiol (2019): 48-58

Viral Regulation on Bacterial Community Impacted by Lysis-Lysogeny Switch: A Microcosm Experiment in Eutrophic Coastal Waters
Authors: Chen, X., Ma, R., Yang, Y., Jiao, N., Zhang, R.
Journal: Front Microbiol (2019): 1763

An electro-conductive plane heating element for rapid thermal lysis of bacterial cells
Authors: Kim, Y., Kim, S.
Journal: J Microbiol Methods (2018): 99-103

An improved in-house lysis-filtration protocol for bacterial identification from positive blood culture bottles with high identification rates by MALDI-TOF MS
Authors: Tsuchida, S., Murata, S., Miyabe, A., Satoh, M., Takiwaki, M., Matsushita, K., Nomura, F.
Journal: J Microbiol Methods (2018): 40-45