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- 详细信息
- 文献和实验
- 技术资料
- 英文名:
Cell Meter™ Mitochondrial Autophagy Imaging Kit *Red Fluorescence*
- 库存:
50
- 供应商:
广州市左克生物科技发展有限公司
- 规格:
100Tests
| Ex (nm) | - | Em (nm) | - |
| 分子量 | - | 溶剂 | - |
| 存储条件 | - |
产品基本信息
产品名称:Cell Meter 线粒体自噬荧光成像试剂盒
储存条件:-15℃避光防潮
保质期:24个月
试剂盒成分
组分A:Mitophagy Red 1小瓶
组分B:染色缓冲液1瓶(50毫升)
组分C:DMSO 1小瓶(50 µL)
图2. HeLa细胞与Mitophagy Red 和MitoLite Green FM(Cat#22695)共染色的荧光图像,位于96孔板中。 在添加CCCP(10 uM)1分钟之前(左侧)和之后(右侧)获取图像。 使用荧光显微镜对细胞进行成像,荧光显微镜具有用于Mitophagy Red 的Cy3 / TRITC滤波片和用于MitoLite Green FM的FITC滤波片,并且彼此重叠。
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文献和实验Transformation of E. coli by Electroporation
autoclaved bottles!). 4. Wash the cell pellet by resuspending in 250 ml of sterile ice-cold WB (10% redistilled glycerol, 90% distilled water, v/v). Centrifuge the cell suspension at 5,000 RPM for 15 min and carefully pour off the supernate as soon
. If the solution needs to be at a specific pH, check the pH meter with fresh buffer solutions and follow instructions for using a pH meter. Autoclave, if possible, at 121EC for 20 min. Some solutions cannot be autoclaved, for example, SDS
. Thus, there is a relationship between the voltage and the amount of dye in the sample. Given the geometry of a spectrophotometer, what is actually measured at the photocell is the amount of light energy which arrives at the cell. The voltage meter is reading
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