- ￥3480
- LSM Bio
- 0.78-50ng/ml
- 0.469ng/ml
- 进口
- ELI-30351b
- 2025年07月14日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
100
- 供应商：
LSM BIO
- 检测范围：
0.78-50ng/ml
- 检测方法：
夹心法
- 应用：
利用ELISA方法体外定量检测牛血清，血浆等样本中目标蛋白的浓度
- 适应物种：
牛
- 标记物：
Bovine Gamma-soluble NSF attachment protein,NAPG
- 样本：
牛的血浆，血清，细胞上清等
- 灵敏度：
0.469ng/ml
- 规格：
96tests
Bovine Gamma- soluble NSF attachment protein, NAPG ELISA KIT
Product Name:Bovine Gamma- soluble NSF attachment protein, NAPG ELISA KIT
Packing:96T
Catalog No.:ELI-30351b
Gene Name:Bovine Gamma-soluble NSF attachment protein, NAPG
Detect Range:0.156-10ng/ml
Sensitivity:0.094ng/ml
Target Protein Name:Bovine Gamma-soluble NSF attachment protein, NAPG
Alternative Name:NAPG,Bovine Gamma-soluble NSF attachment protein, NAPG
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Bovine Gamma- soluble NSF attachment protein, NAPG ELISA KIT allows for the in vitro quantitative determination of Bovine Gamma-soluble NSF attachment protein, NAPG concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Bovine Gamma- soluble NSF attachment protein, NAPG ELISA KIT has been pre-coated with an Bovine Gamma-soluble NSF attachment protein, NAPG antibody specific to Bovine Gamma-soluble NSF attachment protein, NAPG .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Bovine Gamma-soluble NSF attachment protein, NAPG and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Bovine Gamma-soluble NSF attachment protein, NAPG, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Bovine Gamma-soluble NSF attachment protein, NAPG in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验(IgG - gamma globulin)is the preferred protein standard.The addition of 1 M NaOH was suggested by Stoscheck (1990)to allow the solubilization of membrane proteins and reduce the protein-to-protein variation in color yield.References•Bradford,MM.A rapid
. The Bradford assay is rather sensitive to bovine serum albumin, more so than "average" proteins, by about a factor of two. Immunoglogin G (IgG - gamma globulin) is the preferred protein standard. The addition of 1 M NaOH was suggested by Stoscheck (1990
【资源】全面归类网址：毕赤酵母表达各种来源蛋白（细菌，真菌，植物，人类等）
[151,152,153, 387]Bovine lysozyme c2S, 550 mg/L, native[147]Bovine mannose 6-phosphate receptorsS, α-MF, 28 mg purify. protein/L517Bovine opsinS*, 0.3 mg/L, PHO1[148]Bovine pancreatic trypsin inhibitor (aprotinin)S, 930 mg/L, α-MF[149]Bovine tissue-type
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