Bovine Fibrinogen alpha chain,

Bovine Fibrinogen alpha chain, FGA ELISA Kit

96 Tests

Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

Synonyms

Fibrinogen alpha chain, FAP,Fibroblast activation protein,FGA, fibroblast activation protein alpha; DPPIV; FAPA; 170 kDa melanoma membrane-bound gelatinase; integral membrane serine protease; seprase

Search name

Bovine Fibrinogen alpha chain ELISA KIT ,Bovine FAP ELISA KIT ,Bovine Fibroblast activation protein ELISA KIT ,Bovine FGA ELISA KIT ,Bovine fibroblast activation protein alpha ELISA KIT ,Bovine DPPIV ELISA KIT ,Bovine FAPA ELISA KIT ,Bovine 170 kDa melanoma membrane-bound gelatinase ELISA KIT ,Bovine integral membrane serine protease ELISA KIT ,Bovine seprase ELISA KIT

Intended use

This immunoassay kit allows for the in vitro quantitative determination of bovine fibrinogen alpha chain,FGA concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to FGA. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for FGA and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain FGA, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of FGA in the samples is then determined by comparing the O.D. of the samples to the standard curve.

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