- ￥2262
- LSM Bio
- 进口
- PAab07106
- 2025年07月15日
- ELISA,WB,IHC,IF,FC
- E. coli - derived recombinant protein
- Human,Mouse ,Rat
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Ran GTPase activating protein 1
- 亚型：
IgG
- 形态：
liquid
- 保存条件：
负20摄氏度
- 克隆性：
Polyclonal antibody
- 标记物：
Non-conjugated
- 适应物种：
Human,Mouse ,Rat
- 保质期：
6个月
- 抗原来源：
Rabbit
- 目录编号：
P67936
- 级别：
纯化级别
- 库存：
50
- 供应商：
LSM bio
- 宿主：
E. coli - derived recombinant protein
- 应用范围：
ELISA,WB,IHC,IF,FC
- 浓度：
≥95% as determined by SDS-PAGE
- 靶点：
Ran GTPase activating protein 1
- 抗体英文名：
anti-RANGAP1 antibody,RANGAP1 antibody
- 抗体名：
anti-RANGAP1 抗体,RANGAP1 抗体
- 规格：
100μg
RANGAP1抗体| RANGAP1 antibody
货号 PAab07106
蛋白别名 KIAA1835 antibody, SD
蛋白介绍
GTPase activator for the nuclear Ras-related regulatory protein Ran, converting it to the putatively inactive GDP-bound state.
产品描述
anti-RANGAP1 antibody is a Rabbit Polyclonal antibody againstRANGAP1..
建议稀释比例
IHC
Immunohistochemistry of paraffin-embedded human gliomas using PAab07106(RANGAP1 antibody) at dilution of 1:100
Western blot
HEK-293 cells were subjected to SDS PAGE followed by western blot with PAab07106(RANGAP1 antibody) at dilution of 1:1000(本抗体仅供体外科研用途，不可用于临床诊断!) "">
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文献和实验An In Vitro FRET-Based Assay for the Analysis of SUMO Conjugation and Isopeptidase Cleavage
and purifi ed YFP-SUMO-1 and CFP-RanGAP1 as model substrates that are covalently coupled in the presence of recombinant SUMO E1 and E2 enzymes and ATP. Reactions of 25 μl volume, set up in 384-wells plates, give suffi cient signal for analysis. Consequently
Performing In Vitro Sumoylation Reactions Using Recombinant Enzymes
for in vitro sumoylation of RanGAP1 and Sp100 in the absence or presence of the SUMO E3 ligase RanBP2, respectively, are provided. Both protocols can be adapted easily to test in vitro conjugation of other target proteins and/or E3 ligases.
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