- ￥2262
- LSM Bio
- 进口
- PAab07089
- 2025年07月16日
- ELISA,WB,IHC,IF,FC,IP
- E. coli - derived recombinant protein
- Human, Mouse
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
recombination activating gene 2
- 亚型：
IgG
- 形态：
liquid
- 保存条件：
负20摄氏度
- 克隆性：
Polyclonal antibody
- 标记物：
Non-conjugated
- 适应物种：
Human, Mouse
- 保质期：
6个月
- 抗原来源：
Rabbit
- 目录编号：
P67936
- 级别：
纯化级别
- 库存：
50
- 供应商：
LSM bio
- 宿主：
E. coli - derived recombinant protein
- 应用范围：
ELISA,WB,IHC,IF,FC,IP
- 浓度：
≥95% as determined by SDS-PAGE
- 靶点：
recombination activating gene 2
- 抗体英文名：
anti-RAG2 antibody,RAG2 antibody
- 抗体名：
anti-RAG2 抗体,RAG2 抗体
- 规格：
100μg
RAG2抗体| RAG2 antibody
货号 PAab07089
蛋白别名
蛋白介绍
Core component of the RAG complex, a multiprotein complex that mediates the DNA cleavage phase during V(D)J recombination. V(D)J recombination assembles a diverse repertoire of immunoglobulin and T-cell receptor genes in developing B and T- lymphocytes through rearrangement of different V (variable), in some cases D (diversity), and J (joining) gene segments. DNA cleavage by the RAG complex occurs in 2 steps: a first nick is introduced in the top strand immediately upstream of the heptamer, generating a 3'-hydroxyl group that can attack the phosphodiester bond on the opposite strand in a direct transesterification reaction, thereby creating 4 DNA ends: 2 hairpin coding ends and 2 blunt, 5'-phosphorylated ends. The chromatin structure plays an essential role in the V(D)J recombination reactions and the presence of histone H3 trimethylated at 'Lys-4' (H3K4me3) stimulates both the nicking and haipinning steps. The RAG complex also plays a role in pre-B cell allelic exclusion, a process leading to expression of a single immunoglobulin heavy chain allele to enforce clonality and monospecific recognition by the B- cell antigen receptor (BCR) expressed on individual B-lymphocytes. The introduction of DNA breaks by the RAG complex on one immunoglobulin allele induces ATM-dependent repositioning of the other allele to pericentromeric heterochromatin, preventing accessibility to the RAG complex and recombination of the second allele. In the RAG complex, RAG2 is not the catalytic component but is required for all known catalytic activities mediated by RAG1. It probably acts as a sensor of chromatin state that recruits the RAG complex to H3K4me3 (By similarity).
产品描述
anti-RAG2 antibody is a Rabbit Polyclonal antibody againstRAG2..
建议稀释比例
IHC
Immunohistochemistry of paraffin-embedded human lymphoma using PAab07089(RAG2 antibody) at dilution of 1:50
Western blot
A375 cells were subjected to SDS PAGE followed by western blot with PAab07089(RAG2 antibody) at dilution of 1:300(本抗体仅供体外科研用途，不可用于临床诊断!) "">
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文献和实验Production and Characterization of Humanized Rag2/c/ Mice
engraftment that is detectable in many mouse organs, and the ability to generate de novo human innate and adaptive immune responses. Here, we describe the methods used to produce and characterize humanized Rag2−/− γc−/− mice.
Characterization of HIV-1 Infection in the Humanized Rag2/c/ Mouse Model
Engraftment of immunodeficient mice with a human immune system (humanized mice) provides a model system to study pathogens that target human immune cells. Humanized Rag2−/− γc−/− mice produce the major target cells of HIV-1 and these cells
is related to metastatic spread of the disease. As a result NK cell activity directly influences treatment options and mortality. Newly developed immunodeficient mouse strains lacking functional T- and B-cells (rag2 knockout) however presenting functional NK
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